Derivation of oocyte-like cells from a clonal pancreatic stem cell line
Derivation of oocyte-like cells from a clonal pancreatic stem cell line
复制标题
DOI：
10.1093/molehr/gal096
复制
发表时间：
2007-01-01
影响因子：
4
通讯作者：
Kruse, C.
中科院分区：
文献类型：
--
作者：
Danner, S.;Kajahn, J.;Kruse, C.
成体胰腺干细胞（PSC）能够在体外自发分化为各种体细胞类型。从大鼠胰腺中分离的干细胞显示出广泛的自我更新能力，并在高活力的长期培养中生长。此外，这些细胞表达典型的干细胞标志物，例如Oct-4、巢蛋白和SSEA-1。尽管在长期培养中分化潜力略有下降，但可以将细胞系保持至第140代。不同代次的细胞均可建立克隆细胞系，并表现出相似的特性。值得注意的是，从第75代产生的一个克隆细胞系在进一步培养期间显示出异常特性。克隆细胞形成聚集体，在悬浮培养中形成组织样结构。这些生成的3D聚集体在外侧边缘产生永久性的新细胞。释放的细胞有显着的大小，并通过光学显微镜分析仔细检查显示卵母细胞样形态。第8代和第75代原代培养物、克隆细胞系和从组织样结构产生的卵母细胞样细胞（OLC）之间的基因表达模式的比较显示出一些差异。在克隆细胞系中，各种生殖细胞标志物（如Vasa、生长分化标志物9和SSEA-1）的表达增加，并且OLC还显示出减数分裂特异性标志物SCP 3和DMC 1的表达。我们在这里提出了第一个试点研究调查成人PSC的生殖细胞潜力。
Adult pancreatic stem cells (PSCs) are able to differentiate spontaneously in vitro into various somatic cell types. Stem cells isolated from rat pancreas show extensive self-renewal ability and grow in highly viable long-term cultures. Additionally, these cells express typical stem cell markers such as Oct-4, nestin and SSEA-1. Although differentiation potential is slightly decreasing in long-term cultures, it is possible to keep cell lines up to passage 140. Clonal cell lines could be established from different passages and showed similar characteristics. Remarkably, one clonal cell line, generated from passage 75, showed deviant properties during further culture. Clonal cells formed aggregates, which built tissue-like structures in suspension culture. These generated 3D aggregates produced permanently new cells at the outside margin. Released cells had remarkable size, and closer examination by light microscopy analysis revealed oocyte-like morphology. A comparison of the gene expression patterns between primary cultures of passages 8 and 75, the clonal cell line and the produced oocyte-like cells (OLCs) from tissue-like structures demonstrated some differences. Expression of various germ cell markers, such as Vasa, growth differentiation marker 9 and SSEA-1, increased in the clonal cell line, and OLCs showed additionally expression of meiosis-specific markers SCP3 and DMC1. We here present a first pilot study investigating the putative germ line potential of adult PSCs.