Effect of PEF Pre-Treatment and Extraction Temperature on the Recovery of Carotenoids from Tomato Wastes
Effect of PEF Pre-Treatment and Extraction Temperature on the Recovery of Carotenoids from Tomato Wastes
复制标题
PEF预处理和提取温度对番茄废弃物中类胡萝卜素回收的影响
DOI：
10.3303/cet1975024
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
F. Giovanna
中科院分区：
文献类型：
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作者：
G. Pataro;D. Carullo;F. Giovanna
研究了脉冲电场(PEF)前处理和提取温度对水蒸气漂白整果番茄果皮中类胡萝卜素回收率的影响。在不同场强(E=0.5~5kV/cm)和总比能量输入(WT=0.5~20kJ/kg)下进行PEF预处理。以细胞崩解指数(ZP)为指标，确定了番茄果皮在不同温度(20~50℃)丙酮浸提前的最佳脉冲电场处理条件。对未经处理和PEF处理的样品的提取物进行了类胡萝卜素的含量和组成以及抗氧化能力的分析。根据ZP值，在所研究的任何场强下，5kJ/kg的能量输入足以达到番茄果皮组织的最大透过率。在此最佳能量输入下，只有经5kV/cm脉冲电场处理的果皮提取物的总类胡萝卜素含量(47.3%)和抗氧化力(68%)显著高于未处理的样品。无论采用PEF预处理法，提高提取温度都不会进一步提高萃取物中类胡萝卜素的回收率。此外，高效液相色谱法分析表明，番茄红素是提取的类胡萝卜素的主要成分，没有发生降解/异构化现象。研究结果表明，番茄加工废弃物的PEF预处理法与室温或中等提取温度相结合，可以为番茄加工链增加新的价值，提高经济效益，减少浪费问题。
In this work, the influence of pulsed electric fields (PEF) pre-treatment and extraction temperature on the recovery of carotenoid compounds from tomato peels, achieved after peeling of steam blanched whole tomato fruits, was investigated. PEF pre-treatments were carried out at different field strengths (E = 0.5–5 kV/cm) and total specific energy input (WT = 0.5-20 kJ/kg). The cell disintegration index (ZP) was used to identify the optimal PEF processing conditions for the pre-treatment of tomato peels before the extraction with acetone at different temperatures (20-50°C). Extracts from untreated and PEF treated samples were analysed in terms of content and composition of carotenoids and antioxidant power. According to the ZP values, an energy input of 5 kJ/kg was enough for reaching the maximal permeabilization of tomato peel tissues at any investigated field strength. At this optimal energy input, only the extracts obtained from PEF pre-treated peels at 5 kV/cm showed a significantly higher total carotenoids content (47.3%) and antioxidant power (68%), as compared to the untreated samples. Regardless of the application of a PEF pre-treatment, the increase of the extraction temperature did not lead to further intensification in the recovery yield of total carotenoids in the extracts. Moreover, HPLC analyses revealed that lycopene was the main carotenoid extracted and no degradation/isomerization phenomena occurred. Results obtained in this work demonstrated that the PEF pretreatment of tomato processing wastes coupled with room or moderate extraction temperature could add new value to the tomato processing chain, improving economic performances and decreasing waste problems.