GLUTATHIONE AND GLUTATHIONE S-TRANSFERASES IN BARRETT EPITHELIUM
GLUTATHIONE AND GLUTATHIONE S-TRANSFERASES IN BARRETT EPITHELIUM
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DOI：
10.1038/bjc.1993.262
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发表时间：
1993-06-01
影响因子：
8.8
通讯作者：
JANSEN, JBMJ
中科院分区：
文献类型：
--
作者：
PETERS, WHM;ROELOFS, HMJ;JANSEN, JBMJ
对10名Barrett食管患者的正常食管上皮和Barrett食管上皮中的谷胱甘肽含量、酶活性和谷胱甘肽S-转移酶同工酶组成进行了测定。此外，还对同一患者的胃和十二指肠标本进行了研究。与正常食管粘膜相比，Barrett上皮中的谷胱甘肽含量、谷胱甘肽S-转移酶活性以及谷胱甘肽S-转移酶pi含量均显着降低。相反，谷胱甘肽S-转移酶类α酶在巴雷特上皮中显着表达，而在正常食管上皮中仅存在少量。 Barrett 上皮中的谷胱甘肽和谷胱甘肽 S-转移酶组成与胃上皮具有惊人的相似性，而十二指肠上皮则具有相当高含量的谷胱甘肽和谷胱甘肽 S-转移酶，但 pi 类谷胱甘肽 S-转移酶的水平较低。肿瘤的发生率。由于谷胱甘肽和谷胱甘肽 S-转移酶与细胞或细胞遗传学损伤的保护相关，巴雷特食管中谷胱甘肽和谷胱甘肽 S-转移酶的低含量可能是该组织中肿瘤风险增加的相关因素。
Glutathione content, enzyme activity and isoenzyme composition of glutathione S-transferases were assayed in normal and Barrett's esophageal epithelium of ten patients with Barrett's esophagus. In addition, gastric and duodenal specimens from the same patients were also investigated.Glutathione content, glutathione S-transferase enzyme activity as well as glutathione S-transferase pi content were all significantly lower in Barrett's epithelium as compared to normal esophageal mucosa. In contrast, glutathione S-transferase class alpha enzymes are markedly expressed in Barrett's epithelium, whereas only low amounts are present in normal esophageal epithelium. Glutathione and glutathione S-transferase composition in Barrett's epithelium show striking similarities with gastric epithelium, whereas duodenal epithelium is provided with considerable higher amounts of glutathione and glutathione S-transferases, except for levels of glutathione S-transferase class pi, which are lower.A significant negative correlation exists between glutathione S-transferase enzyme activity in the mucosa along the gastrointestinal tract, and the tumour incidence. Since glutathione and glutathione S-transferase are correlated with protection against cellular or cytogenetic damage, the low content of glutathione and glutathione S-transferases in the Barrett's esophagus may be a factor of relevance for the increased tumour risk in this tissue.