The cotton rat model for adenovirus ocular infection: antiviral activity of cidofovir
The cotton rat model for adenovirus ocular infection: antiviral activity of cidofovir
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DOI：
10.1016/j.antiviral.2003.08.003
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发表时间：
2004-01-01
影响因子：
7.6
通讯作者：
Suzutani, T
中科院分区：
文献类型：
--
作者：
Kaneko, H;Mori, S;Suzutani, T
为了确定化合物对眼部腺病毒（AdV）感染的抗病毒作用，我们在棉鼠眼中建立了 AdV 感染的动物模型。棉鼠眼睛基质内和局部接种四种 AdV 血清型 4、5、8 和 37，并用 1% HPMPC（西多福韦）滴眼液局部治疗，每天两次。提取并匀浆受感染的角膜，并通过噬菌斑测定法测定角膜标本中的病毒滴度。接种AdV 5型的眼睛中的病毒滴度在接种后第0天至第3天达到峰值，并在18.0+/-2.8天内检测到病毒脱落。通过免疫荧光染色在角膜上皮细胞中证实了受感染角膜中的AdV 5抗原。然而，对于 AdV 血清型 4、8 和 37，没有发现病毒在棉鼠眼中持续复制的证据。与对照组相比，感染 AdV 5 的接受西多福韦治疗的眼睛样本显示，平均病毒滴度（第 3-15 天）（P = 0.028）和病毒脱落持续时间（P = 0.0014）有统计显着性降低。 (C) 2003 Elsevier B.V. 保留所有权利。
To determine the antiviral effects of compounds against ocular adenovirus (AdV) infection, we established an animal model of AdV infection in cotton rat eyes. Cotton rat eyes were inoculated intrastromally and topically with four AdV serotypes 4, 5, 8, and 37, and treated topically with 1% HPMPC (cidofovir) eye drops twice a day. The infected corneas were extracted and homogenized, and virus titers in the cornea specimens were determined by a plaque assay. The virus titer in AdV type 5-inoculated eyes peaked on days 0 through 3 after inoculation and virus shedding was detected for 18.0 +/- 2.8 days. AdV 5 antigen in the infected corneas was demonstrated in the corneal epithelial cells by immunofluorescence stain. However, for AdV serotypes 4, 8, and 37, no evidence of continued virus replication in cotton rat eyes was noted. Specimens from cidofovir-treated eyes infected with AdV 5 demonstrated a statistically significant reduction in the mean virus titer (days 3-15) (P = 0.028) and virus shedding duration (P = 0.0014), as compared with those of the control group. (C) 2003 Elsevier B.V. All rights reserved.