NARROW AND ROLLED LEAF 2 regulates leaf shape, malefertility, and seed size in rice
NARROW AND ROLLED LEAF 2 regulates leaf shape, malefertility, and seed size in rice
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窄叶和卷叶 2 调节水稻的叶形、雄性受精性和种子大小
DOI：
10.1111/jipb.12503
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发表时间：
2016
影响因子：
11.4
通讯作者：
Tan Lubin
中科院分区：
文献类型：
--
作者：
Zhao Shuangshuang;Zhao Lei;Liu Fengxia;Wu Yongzhen;Zhu Zuofeng;Sun Chuanqing;Tan Lubin
水稻（Oryza sativa L.）与叶和花的发育密切相关。水稻叶、花粉和种子发育的协调调控是一个重要的生物学和农业问题。在这里，我们鉴定了两个具有窄叶和半卷叶的等位基因水稻突变体，命名为窄叶和卷叶2 - 1（nrl 2 - 1）和nrl 2 - 2。基于图位的分子克隆显示，NRL 2编码一种新的蛋白质，其生物学功能尚不清楚。NRL 2突变体引起多效效应，包括纵脉数目减少、厚壁细胞远轴分化缺陷、绒毡层退化和小孢子发育异常，以及形成比野生型（WT）更细长的种子。NRL 2蛋白与Rolling-leaf（RL 14）相互作用，导致thenr 12突变体的叶片具有比WT更高的纤维素含量和更低的木质素含量，这可能与厚壁细胞分化和绒毡层退化有关。因此，该基因是控制基本细胞和发育过程的重要发育调节因子，可作为高产水稻品种的潜在育种目标。
Grain yield in rice (Oryza sativaL.) is closely related to leaf and flower development. Coordinative regulation of leaf, pollen, and seed development in rice as a critical biological and agricultural question should be addressed. Here we identified two allelic rice mutants with narrow and semi‐rolled leaves, namednarrow and rolled leaf 2‐1(nrl2‐1) andnrl2‐2. Map‐based molecular cloning revealed thatNRL2encodes a novel protein with unknown biochemical function. The mutation ofNRL2caused pleiotropic effects, including a reduction in the number of longitudinal veins, defective abaxial sclerenchymatous cell differentiation, abnormal tapetum degeneration and microspore development, and the formation of more slender seeds compared with the wild type (WT). The NRL2 protein interacted with Rolling‐leaf (RL14), causing the leaves of thenrl2mutants to have a higher cellulose content and lower lignin content than the WT, which may have been related to sclerenchymatous cell differentiation and tapetum degeneration. Thus, this gene is an essential developmental regulator controlling fundamental cellular and developmental processes, serving as a potential breeding target for high‐yielding rice cultivars.