Screening of Serum Biomarkers and Establishment of a Decision Tree in Silica-Exposed Populations by Surface-Enhanced Laser Desorption Ionization Time-of-Fly Mass Spectrometry
Screening of Serum Biomarkers and Establishment of a Decision Tree in Silica-Exposed Populations by Surface-Enhanced Laser Desorption Ionization Time-of-Fly Mass Spectrometry
复制标题
通过表面增强激光解吸电离飞行时间质谱法筛选二氧化硅暴露人群的血清生物标志物并建立决策树
DOI：
10.1097/jom.0b013e318058aa16
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发表时间：
2007
影响因子：
3.2
通讯作者：
Zi
中科院分区：
文献类型：
--
作者：
Shi;Xue;Mao;You Wu;Jia;Xian;Jian;Bing He;Zi
目的：目的应用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）技术筛选矽肺患者血清中的生物标志物并研究其作用。研究方法：采用SELDI-TOF-MS技术，在CM 10蛋白质芯片上采集符合国家尘肺诊断标准的工人血清蛋白/肽谱。使用蛋白质/肽峰进行判别分析以建立决策树。结果：蛋白质/肽峰在二氧化硅暴露人群中的变化比对照组大得多。用Wilks λ法进行判别分析，在区分矽尘暴露人群和对照人群方面具有较高的敏感性和特异性;决策树在区分对照人群中的矽尘病期方面具有100%的敏感性和95%的特异性。结论：矽尘作业工人体内可能存在特异性蛋白质/肽的变化，这些变化可用于矽尘作业人群与对照人群的判别分析。
Objective: To screen for serum biomarkers of silicosis and to study their roles using surface-enhanced laser desorption ionization time-of-fly mass spectrometry (SELDI-TOF-MS) techniques. Methods: The serum protein/peptide profile on the CM10 proteinchip was acquired using SELDI-TOF-MS from workers classified by the Chinese national diagnostic standard for pneumoconiosis. Discriminant analysis was performed to establish a decision tree using protein/peptide peaks. Results: Protein/peptide peaks changed much more in silica-exposed populations than they did in the control. Discriminant analysis using Wilks' lambda method could give high sensitivity and specificity in distinguishing the silica-exposed population from the control; the decision tree could give 100% sensitivity and 95% specificity in distinguishing the silicosis phase among the control group. Conclusions: Special proteins/peptides may change in silica-exposed workers and these changes may be used to distinguish silica-exposed populations from the control with the proper discriminant analytical method.