Characterization of TROY/TNFRSF19/TAJ-expressing cells in the adult mouse forebrain
Characterization of TROY/TNFRSF19/TAJ-expressing cells in the adult mouse forebrain
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DOI：
10.1016/j.brainres.2006.06.068
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发表时间：
2006-09-19
期刊：
影响因子：
2.9
通讯作者：
Senba, Emiko
中科院分区：
文献类型：
--
作者：
Hisaoka, Tomoko;Morikaw, Yoshihiro;Senba, Emiko
肿瘤坏死因子受体超家族（TNFRSF）的成员TROY/TNFRSF 19/TAJ在成年小鼠的脑中高度表达。北方印迹分析表明，从成年人中枢神经系统的区域，包括嗅球，大脑皮层，纹状体和海马的所有区域中的表达TROY。原位杂交和免疫组化结果显示，TROY mRNA和蛋白在成年小鼠的嘴侧迁移流（RMS）和脑室下区（SVZ）有强表达。在成人SVZ中，一些胶质细胞酸性蛋白（GFAP）阳性细胞（B型细胞）被认为是多能神经干细胞。这些B型细胞在表皮生长因子（EGF）存在下缓慢分裂并产生表皮生长因子受体（EGFR）阳性的转运扩增前体细胞（C型细胞）。C型细胞产生神经元特异性III类β-微管蛋白（TuJ 1）阳性神经母细胞（A型细胞），其沿着RMS迁移至嗅球沿着。TROY表达细胞GFAP阳性，EGFR阳性，TuJ 1阴性的成人SVZ。从这些发现中，TROY似乎在B型和C型细胞中表达，但在A型细胞中不表达，这得到免疫电镜的支持。此外，TROY在不同区域如大脑皮层、纹状体和海马的GFAP阳性星形胶质细胞中表达。因此，TROY在未定型的前体细胞和星形胶质细胞谱系细胞中表达，表明TROY在成年CNS中的胶质细胞发生的调节中起一定作用。(c)2006 Elsevier B. V.保留所有权利。
A member of the tumor necrosis factor receptor superfamily (TNFRSF), TROY/TNFRSF19/TAJ, is highly expressed in the brain of adult mice. Northern blot analysis using mRNA taken from regions of the adult CNS showed the expression of TROY in all regions examined, including the olfactory bulb, cerebral cortex, striatum, and hippocampus. In situ hybridization and immunohistochemistry revealed that TROY mRNA and protein were strongly expressed in the rostral migratory stream (RMS) and subventricular zone (SVZ) of adult mice. In the adult SVZ, some glial fibrillary acidic protein (GFAP)-positive cells (type B cells) are thought to be multipotent neural stem cells. These type B cells divide slowly and generate epidermal growth factor receptor (EGFR)-positive transit-amplifying precursor cells (type C cells) in the presence of epidermal growth factor (EGF). Type C cells give rise to neuron-specific class III beta-tubulin (TuJ1)-positive neuroblasts (type A cells) that migrate to the olfactory bulb along the RMS. TROY-expressing cells were GFAP-positive, EGFR-positive, and TuJ1-negative in the adult SVZ. From these findings, TROY appears to be expressed in type B and type C cells, but not in type A cells, which was supported by immunoelectron microscopy. in addition, TROY was expressed in GFAP-positive astrocytes of the various regions, such as the cerebral cortex, striatum, and hippocampus. Thus, TROY was expressed in uncommitted precursor cells and astroglial lineage cells, suggesting that TROY plays some roles in the regulation of gliogenesis in the adult CNS. (c) 2006 Elsevier B.V. All rights reserved.