MicroRNA profiling in the left atrium in patients with non-valvular paroxysmal atrial fibrillation.
MicroRNA profiling in the left atrium in patients with non-valvular paroxysmal atrial fibrillation.
复制标题
非瓣膜性阵发性心房颤动患者左心房的 MicroRNA 分析
DOI：
10.1186/s12872-015-0085-2
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发表时间：
2015-08-29
影响因子：
2.1
通讯作者：
Yang B
中科院分区：
文献类型：
--
作者：
Wang J;Song S;Xie C;Han J;Li Y;Shi J;Xin M;Wang J;Luo T;Meng X;Yang B
背景我们的目的是鉴定左心耳中的miRNA表达谱，旨在鉴定与非瓣膜性阵发性AF显着相关的miRNA。方法从健康对照组（n = 5）和房颤患者（n = 8）中分离RNA样本。为了证实通过分析miRNA谱获得的结果，我们测量了选择的miRNA在整个队列中的表达通过定量PCR.Results10个特定的miRNA被发现在心房颤动和健康对照之间的差异表达超过2倍的变化（P < 0.05）。与所获得的数据相一致，miRNA-155、miRNA-146 b-5 p和miRNA-19 b的表达水平在房颤患者中显著增加。有趣的是，已知与炎症相关的miRNA-146 b-5 p和miRNA-155的水平与左心房尺寸、房颤持续时间和高敏C反应蛋白水平独立且正相关。利用TargetScan、米兰达、Starbase Clip-seq和miRDB四个数据库进行靶基因预测，发现4个基因与炎症反应和纤维化相关，另外3个基因编码心脏离子通道蛋白。作为TaqMan qPCR和Western分析的结果，三个靶基因的相对mRNA和蛋白质表达水平分别为：结论非瓣膜性阵发性心房颤动患者左心耳炎症相关miRNAs表达明显上调，这可能在电和结构重塑中起重要作用。
BackgroundWe aimed to identify the miRNA expression profiles in left atrial appendage, with the intention of identifying miRNAs that were significantly associated with non-valvular paroxysmal AF.MethodsThe RNA samples were isolated from healthy controls (n = 5) and patients with atrial fibrillation (n = 8). To confirm the findings obtained by analyzing the miRNA profile, we measured the expression of selected miRNAs in the entire cohort by quantitative PCR.ResultsTen specific miRNAs were found to be differentially expressed between atrial fibrillation and healthy controls with more than a 2-fold change (P < 0.05). Consistent with the data obtained for the profile, expression levels of miRNA-155, miRNA-146b-5p and miRNA-19b were significantly increased in patients with atrial fibrillation. Interestingly, levels of miRNA-146b-5p and miRNA-155, which are known to be associated with inflammation, were independently and positively associated with left atrium dimension, atrial fibrillation duration and high sensitivity C-reactive protein levels. By using four Databases (TargetScan, miRanda, Starbase Clip-seq and miRDB) to perform target gene prediction, there were four genes were related to the inflammatory response and fibrosis, and three others encoding cardiac ion channel proteins. As a result of TaqMan qPCR and Western analysis, the relative mRNA and protein expression level of three target genes (DIER-1, TIMP-4 and CACNA1C) were significantly lower in the atrial fibrillation group than that in the healthy control group.ConclusionsExpression of inflammation-associated miRNAs is significantly up-regulated in the left atrial appendage of patients with non-valvular paroxysmal atrial fibrillation, which may play a significant role in electrical and structural remodeling.