Induction of adrenomedullin by hypoxia in cultured human coronary artery endothelial cells
Induction of adrenomedullin by hypoxia in cultured human coronary artery endothelial cells
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DOI：
10.1016/s0196-9781(99)00061-3
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发表时间：
1999-01-01
期刊：
影响因子：
3
通讯作者：
Shibahara, S
中科院分区：
文献类型：
--
作者：
Nakayama, M;Takahashi, K;Shibahara, S
为探讨肾上腺髓质素(adrenomedullin，ADM)在缺血性心脏病病理生理机制中的作用，我们观察了低氧对培养的人冠状动脉内皮细胞产生和分泌ADM的影响。低氧(5%CO2/94%N-2/1%O-2)处理6h和12h后，ADM mRNA的表达水平分别是常氧对照组的2.2倍和5倍。缺氧12h培养上清液中ADM免疫反应阳性细胞数为对照组的5倍(缺氧时每12h增加39.0+/-1.1fmol/10(5)个细胞，常氧下每12h增加7.9+/-0.4fmol/10(5)个细胞；P<；0.01，n=4，均值+/-SEM)。常氧和低氧条件下培养基萃取物的反相高效液相色谱显示，在人ADM标准品位置有一个主峰洗脱。缺氧条件下培养的人冠状动脉内皮细胞产生和分泌ADM增多，ADM可能在缺血性心脏病中起重要的病理生理作用。(C)1999 Elsevier Science Inc.保留所有权利。
To explore the role of adrenomedullin (ADM) in pathophysiology of ischemic heart disease, we investigated the effects of hypoxia on the production and secretion of ADM in cultured human coronary artery endothelial cells. Treatment with hypoxia (5% CO2/94% N-2/1% O-2) for 6 and 12 h increased expression levels of ADM mRNA 2.2-fold and fivefold compared with the normoxia control, respectively. The levels of immunoreactive ADM in the media were increased by 12-h hypoxia about fivefold compared with the control (39.0 +/- 1.1 fmol/10(5) cells per 12 h under hypoxia and 7.9 +/- 0.4 fmol/10(5) cells per 12 h under normoxia; P < 0.01, n = 4, mean +/- SEM). Reverse-phase high-performance liquid chromatography of the extracts of culture media under normoxia and hypoxia showed one major peak eluting in the position of human ADM standard. The production and secretion of ADM were increased in cultured human coronary artery endothelial cells under hypoxia, ADM may therefore play an important pathophysiological role in ischemic heart disease. (C) 1999 Elsevier Science Inc. All rights reserved.