A hydrophobic organelle probe based on aggregation-induced emission: Nanosuspension preparation and direct use for endoplasmic reticulum imaging in living cells.
A hydrophobic organelle probe based on aggregation-induced emission: Nanosuspension preparation and direct use for endoplasmic reticulum imaging in living cells.
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DOI：
10.1016/j.saa.2017.08.016
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发表时间：
2018-01
期刊：
影响因子：
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通讯作者：
Sichao Zheng;Cuihong Huang;Xuyan Zhao;Yong Zhang;Shuwen Liu;Qiuhua Zhu
中科院分区：
文献类型：
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作者：
Sichao Zheng;Cuihong Huang;Xuyan Zhao;Yong Zhang;Shuwen Liu;Qiuhua Zhu
有机荧光团具有广泛的生物用途，并且由于其疏水性质，通常需要制备成水溶性化合物或纳米颗粒以用于水性生物系统中，这通常是一个复杂、耗时且高成本的过程。在这里，疏水荧光团的纳米颗粒的制备及其在细胞成像中的应用进行了研究。结果发现：a）胎牛血清（FBS）对疏水性小分子有机化合物显示出优异的分散效果; B）疏水性C6-未取代的四氢嘧啶（Me-THP-Naph）可以利用具有10%FBS的细胞培养基制备为纳米悬浮液，并直接用作内质网（ER）的特异性实时成像探针，内质网是在许多细胞过程中起关键作用的动态细胞器。与现有的ER靶向有机荧光探针相比，我们开发的五组分反应产物Me-THP-Naph具有非传统的聚集诱导发光特性，具有成本低、染色时间长、光稳定性好、信噪比高和生物相容性好等优点，有望成为ER实时成像的特异性探针。更重要的是，这项工作提供了一个简单的策略，疏水有机化合物在水相生物系统中的直接应用。
Organic fluorophores have a wide range of biological uses and are usually needed to be prepared as water-soluble compounds or nanoparticles for applications in aqueous biosystems owing to their hydrophobic properties, which often is a complex, time-consuming and high-cost process. Here, the nanoparticle preparation of hydrophobic fluorophores and their application in cell imaging have been investigated. It was found: a) fetal bovine serum (FBS) shows an excellent dispersion effect on hydrophobic small-molecule organic compounds; b) a hydrophobic C6-unsubstituted tetrahydropyrimidine (Me-THP-Naph) can be prepared as nanosuspensions utilizing cell culture medium with 10% FBS and directly be used as a specific real-time imaging probe for the endoplasmic reticulum (ER), a dynamic organelle playing a crucial role in many cellular processes. Compared with existing ER-targeted organic fluorescent probes, Me-THP-Naph, a product of an efficient five-component reaction that we developed, has unconventional aggregation-induced emission characteristics and shows advantages of low cost, long-term staining, good photostability, high signal-to-noise ratio and excellent biocompatibility, which make it a potential specific probe for real-time ER imaging. More importantly, this work affords a simple strategy for direct application of hydrophobic organic compounds in aqueous biological systems.