Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol
Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol
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DOI：
10.1016/s0300-9084(99)80132-1
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发表时间：
1999-07-01
期刊：
影响因子：
3.9
通讯作者：
Desideri, A
中科院分区：
文献类型：
--
作者：
Santucci, R;Polizio, F;Desideri, A
采用圆二色性、吸光度和EPR光谱研究了甘油对细胞色素c结构的影响。结果表明，增加多元醇水溶液中有机溶剂的浓度（70-99.2%,v/v），可使天然细胞色素c通过完全可逆的两态转变转变为一种新的低自旋形式。甘油稳定的形式（这里我们称之为G态）保留了天然的α -螺旋结构，而刚性的三级结构和天然的Fe(III)-Met（80）轴向键则丢失了。数据分析表明G态具有熔球特征；G态的光谱特性（以及结构特性）与蛋白质的酸性熔融球（A态）惊人的相似，为这一观点提供了支持。(C) 1999法国生物化学和生物分子学会/ Elsevier SAS科学和医学版。
The effect of glycerol on the structure of cytochrome c was investigated by circular dichroism, absorbance and EPR spectroscopy. The results obtained show that an increasing concentration of the organic solvent (70-99.2%, v/v) in aqueous polyalcohol mixtures converts native cytochrome c into a new, low spin form through a fully reversible, two-state transition. The glycerol-stabilized form (that we call here the G state) retains native-like amounts of alpha-helix structure while rigid tertiary structure and native Fe(III)-Met(80) axial bond are lost. Analysis of data suggests a molten globule character of the G state; support to this view is afforded by the striking similarities between the spectroscopic (and, thus, structural) properties of the G state with those of the acidic molten globule of the protein (A state). (C) 1999 Societe francaise de biochimie et biologie moleculaire / Editions scientifiques et medicales Elsevier SAS.