Magnetic and pH dual-responsive mesoporous silica nanocomposites for effective and low-toxic photodynamic therapy.
Magnetic and pH dual-responsive mesoporous silica nanocomposites for effective and low-toxic photodynamic therapy.
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磁性和 pH 双重响应介孔二氧化硅纳米复合材料用于有效且低毒的光动力治疗
DOI：
10.2147/ijn.s127528
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发表时间：
2017
影响因子：
8
通讯作者：
Yang F
中科院分区：
文献类型：
--
作者：
Zhan J;Ma Z;Wang D;Li X;Li X;Le L;Kang A;Hu P;She L;Yang F
光动力学疗法（PDT）的非特异性靶向性、大剂量和光毒性严重影响了其临床疗效。本工作制备了pH响应性嵌段聚合物聚乙二醇-b-聚天冬氨酸（PEG-b-PAsp）接枝的超顺磁性Fe 3 O 4介孔二氧化硅纳米粒子，并负载模型光敏剂玫瑰红（RB），以提高PDT的效率。与游离RB相比，纳米复合物（聚乙二醇-b-聚碳酸酯修饰的负载有玫瑰红的磁性介孔二氧化硅[RB-MMSNs]）可以由于其有效的内吞作用而大大增强小鼠黑色素瘤B16细胞的细胞摄取，并且表现出更高的诱导凋亡，尽管几乎没有暗毒性。RB−MMSNs几乎没有暗毒性，甚至可以通过体外磁场促进。细胞计数试剂盒-8（CCK-8）结果和凋亡检测均显示，在相同RB浓度下，RB− MMSN的诱导凋亡效率是游离RB的10倍。此外，RB− MMSN介导的PDT在荷瘤小鼠体内显示出RB− MMSN对肿瘤部位的稳定物理靶向;在磁场中用绿色光照射显著减小了肿瘤体积。更重要的是，用RB− MMSN治疗的荷瘤小鼠的生存时间大大延长。聚乙二醇-b-聚碳酸酯修饰的磁性介孔氧化硅（MMSNs）作为光敏剂囊泡，具有高效、低毒的特点，在临床肿瘤光动力治疗中具有巨大的应用潜力。
Nonspecific targeting, large doses and phototoxicity severely hamper the clinical effect of photodynamic therapy (PDT). In this work, superparamagnetic Fe3O4 mesoporous silica nanoparticles grafted by pH-responsive block polymer polyethylene glycol-b-poly(aspartic acid) (PEG-b-PAsp) were fabricated to load the model photosensitizer rose bengal (RB) in the aim of enhancing the efficiency of PDT. Compared to free RB, the nanocomposites (polyethylene glycol-b-polyaspartate-modified rose bengal-loaded magnetic mesoporous silica [RB−MMSNs]) could greatly enhance the cellular uptake due to their effective endocytosis by mouse melanoma B16 cell and exhibited higher induced apoptosis although with little dark toxicity. RB−MMSNs had little dark toxicity and even much could be facilitated by magnetic field in vitro. RB−MMSNs demonstrated 10 times induced apoptosis efficiency than that of free RB at the same RB concentration, both by cell counting kit-8 (CCK-8) result and apoptosis detection. Furthermore, RB−MMSNs-mediated PDT in vivo on tumor-bearing mice showed steady physical targeting of RB−MMSNs to the tumor site; tumor volumes were significantly reduced in the magnetic field with green light irradiation. More importantly, the survival time of tumor-bearing mice treated with RB−MMSNs was much prolonged. Henceforth, polyethylene glycol-b-polyaspartate-modified magnetic mesoporous silica (MMSNs) probably have great potential in clinical cancer photodynamic treatment because of their effective and low-toxic performance as photosensitizers’ vesicles.