Cytotoxic effects of ionic high-osmolar, nonionic monomeric, and nonionic iso-osmolar dimeric iodinated contrast media on renal tubular cells in vitro
Cytotoxic effects of ionic high-osmolar, nonionic monomeric, and nonionic iso-osmolar dimeric iodinated contrast media on renal tubular cells in vitro
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DOI：
10.1148/radiol.2353040726
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发表时间：
2005-06-01
期刊：
影响因子：
19.7
通讯作者：
Uder, M
中科院分区：
文献类型：
--
作者：
Heinrich, MC;Kuhlmann, MK;Uder, M
目的：比较二聚体和单体碘对比剂对体外培养的肾小管上皮细胞渗透压的影响。材料和方法：将LLC-PK1细胞分别加入碘浓度为18.75~150 mg/毫升的Ioxithalamate、Ioverol、Iomeprol-300、Iomeprol-150、Idixano、IOtrolan和高渗甘露醇溶液孵育1~24小时。用四甲基偶氮唑蓝(3-(4，5-dimethyl-2-thiazolyi)-2，5-diphenyl-2H-tetrazolium)比色法检测细胞毒作用。结果：在相同的碘浓度下，含碘75 mg/毫升的未损伤对照细胞的MTT值分别为4%、34%、40%、41%和41%，差异有统计学意义(P<0.05)。在相同摩尔浓度下，二聚体造影剂的细胞毒作用明显强于低渗透单体造影剂(碘化醇为40%，碘曲兰为41%，而碘佛醇为98.5mmoL/L，碘丙醇为59%，n=61~75，P
PURPOSE: To compare the cytotoxic effects of dimeric and monomeric iodinated contrast media on renal tubular cells in vitro with regard to osmolality.MATERIALS AND METHODS: LLC-PK1 cells were incubated with ioxithalamate, ioversol, iomeprol-300, iomeprol-150, iodixanol, iotrolan, and hyperosmolar mannitol solutions for 1-24 hours at concentrations from 18.75 to 150 mg of iodine per milliliter. Cytotoxic effects were assessed with 3-(4,5-dimethyl-2-thiazolyi)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Data were analyzed with one-way analysis of variance; post hoc tests were performed.RESULTS: At equal iodine concentrations, ioxithalarnate showed stronger cytotoxic effects than did other contrast media (MTT conversion for ioxithalarnate was 4% vs that for ioversol of 32%, that for iomeprol-300 of 34%, that for iodixanol of 40%, and that for iotrolan of 41% of undamaged control cells at 75 mg of iodine per milliliter, n = 61-90, P .05). At equal molarity, dimeric contrast media induced significantly stronger cytotoxic effects than did low-osmolar monomeric contrast media (40% for iodixanol and 41% for iotrolan vs 64% for ioversol and 59% for iomeprol-300 at 98.5 mmol/L, n = 61-75, P