Herpes simplex virus in primary graft failure
Herpes simplex virus in primary graft failure
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DOI：
10.1001/archopht.1997.01100150588001
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发表时间：
1997-05-01
影响因子：
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通讯作者：
McLean, IW
中科院分区：
文献类型：
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作者：
Cockerham, GC;Krafft, AE;McLean, IW
目的：确定原发性角膜移植失败中单纯疱疹病毒1型（HSV-1）的发生频率。方法：对在武装部队病理学研究所（华盛顿，DC）存档的所有角膜移植失败病例的临床资料进行评价。结果：106例移植肾功能衰竭患者中3例（2.8%）为原发性移植肾功能衰竭。3个角膜扣中有2个的DNA可通过PCR分析证实，PCR分析和Southern印迹结果均为HSV-1阳性。30天后发生的3例失败的角膜扣的PCR分析和Southern印迹结果均为HSV-1阳性。PCR分析和Southern印迹结果表明，临床怀疑为单纯疱疹性角膜炎的对照组中，3个角膜按钮中有2个为HSV-1阳性。第一例原发性移植失败的角膜显示急性炎症伴基质坏死。第二例角膜内皮细胞的损失没有inflammation.Conclusions：从HSV-1的DNA在角膜按钮从2例原发性移植失败支持类似的观察Cleator等，并表明HSV-1可能是致病性的原发性移植失败的某些情况下。需要更大规模的研究来确定HSV-1是否是原发性移植物失败的致病因素。
Objective: To determine the frequency of herpes simplex virus type 1 (HSV-1) in primary corneal graft failures.Methods: The clinical data submitted about all cases of corneal graft failure on file at the Armed Forces Institute of Pathology, Washington, DC, from the last 25 years (1970-1995) were evaluated. Cases that met the definition of primary graft failure were examined microscopically and analyzed using polymerase chain reaction (PCR) for the DNA of HSV-1.Results: Three (2.8%) of the 106 cases of graft failure were primary graft failures. The DNA from 2 of the 3 corneal buttons was amplifiable by PCR analysis and results of the PCR analysis and Southern blotting were positive for HSV-1. None of the results of the PCR analysis and Southern blotting of the corneal buttons from the 3 failures occurring later than 30 days were positive for HSV-1. Results of the PCR analysis and Southern blotting indicated that 2 of 3 corneal buttons in the control group of clinically suspected herpetic keratitis were positive for HSV-1. The cornea from the first case of primary graft failure showed acute inflammation with stromal necrosis. The cornea in the second case had loss of endothelium without inflammation.Conclusions: The finding of DNA from HSV-1 in corneal buttons from 2 cases of primary graft failure supports similar observations by Cleator et al and suggests that HSV-1 may be pathogenic in some cases of primary graft failure. A larger study is needed to determine if HSV-1 is a causative factor in primary graft failure.