Stoichiometry of soil extracellular enzyme activity along a climatic transect in temperate grasslands of northern China
Stoichiometry of soil extracellular enzyme activity along a climatic transect in temperate grasslands of northern China
复制标题
中国北方温带草原气候样带土壤细胞外酶活性的化学计量
DOI：
10.1016/j.soilbio.2016.04.008
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发表时间：
2016-07-01
影响因子：
9.7
通讯作者：
Wang, Wei
中科院分区：
文献类型：
--
作者：
Peng, Xiaoqian;Wang, Wei
在全球范围和热带生态系统中，碳(C)、氮(N)和磷(P)的比吸收活动的比率趋于1：1：1。目前还不太清楚这种模式是否适用于温带草原。这种格局是否在不同的土壤深度上保持稳定，以及气候、土壤、非生物和生物因素对土壤胞外酶活性(EEA)化学计量学的影响有多大的相对贡献，这些问题仍然不确定。2013年生长季节，测定了中国北部温带草原气候样带土壤中1种碳源酶(β-1，4-葡萄糖苷酶)、2种N源酶(β-N-乙酰氨基葡萄糖苷酶和亮氨酸氨基肽酶)和1种有机磷源酶(酸性磷酸酶)的潜在活性及其主要影响因素。温带草原0~20 cm土层的酶C：N(0.47)和C：P(0.18)低于热带土壤(C：N，1.83；C：P，0.21；N：P，0.13)，N：P活性比(0.40)高于热带土壤。荒漠草原土壤酶C/N和C/P比值除酶C/N比值外，其余均随土壤深度的增加而降低。而酶N/P值随土层深度的变化不显著。其中，土壤全C、N、P含量在0~10 cm表层土壤EEA和生态酶化学计量学中的变异最大，表明土壤EEA化学计量学在很大程度上受土壤养分化学计量学的控制。土壤因子对下层土壤EEA化学计量学的影响小于表层土壤，土壤非生物因子对土壤EEA化学计量学的影响大于气候和生物因子。我们的结果表明，土壤胞外酶活性比不是动态平衡的，而是资源依赖于土壤和微生物生物量化学计量学。(C)2016爱思唯尔有限公司。保留所有权利。
Ratios of specific carbon (C), nitrogen(N) and phosphorus (P) acquisition activities converged on 1:1:1 at a global scale and in tropical ecosystems. It is less clear if this pattern can be applied in temperate grasslands. The questions of whether the pattern remains stable across different soil depths and what the relative contributions are from the influence of climatic, edaphic abiotic and biotic factors on soil extracellular enzyme activity (EEA) stoichiometry remain uncertain. We measured potential activities of one C-acquiring enzyme (beta-1, 4-glucosidase), two N-acquiring enzymes (beta-N-acetylglucosaminidase and leucine aminopeptidase) and one organic P-acquiring enzyme (acid phosphatase) and major influential factors along a climatic transect in temperate grasslands of northern China during the growing season of 2013. We found lower enzyme C: N (0.47) and C: P (0.18) ratios and a higher enzyme N: P activity ratio (0.40) in 0-20 cm soil depth in temperate grasslands than in tropical soils (C: N, 1.83; C:P, 0.21; N: P, 0.13). The enzyme C: N and C: P ratios decreased with soil depth except for the enzyme C: N ratio in desert steppes. However, there were no significant differences in enzyme N: P ratio with soil depth. Among all the factors, soil total C, N and P contents accounted for the most variation in soil EEA and ecoenzymatic stoichiometry in 0-10 cm surface soil, which implied that soil EEA stoichiometry was largely controlled by soil nutrient stoichiometry. Moreover, edaphic factors had less influence on soil EEA stoichiometry in subsoil than in surface soil and edaphic abiotic factors had a larger effect on soil EEA stoichiometry than climatic and biotic factors. Our results suggest that soil extracellular enzyme activity ratios were not in homeostasis but resource dependent on soil and microbial biomass stoichiometry. (C) 2016 Elsevier Ltd. All rights reserved.