The G alpha subunit Gα8 inhibits proliferation, promotes adhesion and regulates cell differentiation.
The G alpha subunit Gα8 inhibits proliferation, promotes adhesion and regulates cell differentiation.
复制标题
G α 亚基 Gα8 抑制增殖、促进粘附并调节细胞分化。
DOI：
10.1016/j.ydbio.2013.05.001
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发表时间：
2013
影响因子：
2.7
通讯作者：
Janetopoulos,Chris
中科院分区：
文献类型：
--
作者：
Wu,Yuantai;Janetopoulos,Chris
Heterotrimeric G protein-mediated signal transduction plays a pivotal role in both vegetative and developmental stages in the eukaryote Dictyostelium discoideum. Here we describe novel functions of the G protein alpha subunit Gα8 during vegetative and development stages. Gα8 is expressed at low levels during vegetative growth. Loss of Gα8 promotes cell proliferation, whereas excess Gα8 expression dramatically inhibits growth and induces aberrant cytokinesis on substrates in a Gβ-dependent manner. Overexpression of Gα8 also leads to increased cell–cell cohesion and cell–substrate adhesion. We demonstrate that the increased cell–cell cohesion is mainly caused by induced CadA expression, and the induced cell–substrate adhesion is responsible for the cytokinesis defects. However, the expression of several putative constitutively active mutants of Gα8 does not augment the phenotypes caused by intact Gα8. Gα8 is strongly induced after starvation, and loss of Gα8 results in decreased expression of certain adhesion molecules including CsA and tgrC1. Interestingly, Gα8 is preferentially distributed in the upper and lower cup of the fruiting body. Lack of Gα8 decreases the expression of the specific marker of the anterior-like cells, suggesting that Gα8 is required for anterior-like cell differentiation.
