REPLICATION ORIGINS ARE ASSOCIATED WITH TRANSCRIPTION INITIATION SEQUENCES IN THE MITOCHONDRIAL GENOME OF YEAST
REPLICATION ORIGINS ARE ASSOCIATED WITH TRANSCRIPTION INITIATION SEQUENCES IN THE MITOCHONDRIAL GENOME OF YEAST
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DOI：
10.1002/j.1460-2075.1982.tb01282.x
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发表时间：
1982-01-01
期刊：
影响因子：
11.4
通讯作者：
BERNARDI, G
中科院分区：
文献类型：
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作者：
BALDACCI, G;BERNARDI, G
研究了一系列S.在一些实施方案中，本发明涉及具有线粒体基因组的果蝇，所述线粒体基因组由不含基因的短重复单元形成，但是：7个ori序列中的一个，DNA复制的典型起点（ori+突变体）;或部分缺失的ori序列，缺乏富含GC的簇A或C（ori-突变体）;或没有典型ori序列，而只有oriS序列，复制的替代起点（oriO突变体）。显然，一些ori序列在转录起始中起作用，并且簇C的存在，更具体地说，簇C旁边的富含AT的序列的存在，对于转录的发生是必不可少的。用分离的DNA链进行的杂交实验已经鉴定出转录中使用的模板链为含有C簇寡嘧啶段的链。RNA-DNA杂交体的S1降解表明转录起始于C簇寡嘧啶段旁边的TATTACTTATATTT序列，并在C簇→中进行。集群A方向。野生型线粒体基因组的转录这些结果的相关性进行了讨论。
Mitochondrial transcripts were investigated in a series of spotaneous petite mutants of S. cervisiae endowed with mitochondrial genomes formed by short repeat units containing no genes, but either: one of the 7 ori sequences, the canonical origins of DNA replication (ori+ mutants); or partially deleted ori sequences, lacking GC rich clusters A or C (ori- mutants); or no canonical ori sequence, but only oriS sequences, the surrogate origins of replication (oriO mutants). Apparently, some ori sequences play a role in transcription initiation, and that the presence of cluster C and, more specifically, of an AT-rich sequence next to it, are essential for transcription to take place. Hybridizaton experiments with separated DNA strands have identified the template strand used in transcription as the strand containing the oligopyrimidine stretch of cluster C.S1 degradation of RNA-DNA hybrids indicated that transcription initiates at a TATTACTTATATATTT sequence next to the oligopyrimidine stretch of cluster C and proceeds in the cluster C .fwdarw. cluster A direction. The relevance of these results for the transcription of the wild-type mitochondrial genome is discussed.