Morphometric analysis of the developing mouse soleus muscle.
Morphometric analysis of the developing mouse soleus muscle.
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发育中的小鼠比目鱼肌的形态测量分析。
DOI：
10.1002/aja.1001810306
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Bourke,D
中科院分区：
文献类型：
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作者：
Ontell,M;Hughes,D;Bourke,D
使用间隔、连续、超薄切片和计算机辅助形态测量分析对 129 ReJ 小鼠比目鱼肌的器官发生模式进行定量评估。对子宫小鼠 14 天、16 天和 18 天的肌肉以及 1 天和 5 天大的小鼠的肌肉进行分析，以确定肌肉最大周长和长度、肌管数量、簇频率以及肌管长度和直径与年龄相关的变化。子宫内第 14 天时，在肌肉中发现初级肌管。在子宫内 14 至 16 天之间几乎没有从头形成肌管，这一时期主要是初级肌管生长和成熟的时期之一。子宫内 16 至 18 天之间的间隔以广泛的二次肌管形成为标志，在此期间形成的肌管比任何研究时期都多。形态测量数据支持这样的假设：第二代肌管使用初级肌管作为它们形成的支架。形态测量数据还证实了簇形成和簇分散在产前时期同时发生的假设。次级肌管持续形成直至出生。出生时，比目鱼肌含有成人数量的肌纤维。出生后的前 5 天是肌纤维生长和成熟的标志。
The pattern of organogenesis of the soleus muscle of the 129 ReJ mouse was evaluated quantitatively using spaced, serial, ultrathin sections and computer‐assisted morphometric analysis. Muscles from 14‐, 16‐, and 18‐day in utero mice and muscles of 1‐ and 5‐day‐old mice were analyzed to determine age‐related alterations in the maximal girth and length of the muscle, number of myotubes, cluster frequency, and the lengths and diameters of myotubes. Primary myotubes are found in the muscle at 14 days in utero. There is little de novo myotube formation between 14 and 16 days in utero, this interval being principally one of primary myotube growth and maturation. The interval between 16 and 18 days in utero is marked by extensive secondary myotube formation, with more myotubes being formed during this period than in any period studied. Morphometric data support the hypothesis that secondary generation myotubes use primary myotubes as a scaffold on which they are formed. Morphometric data also confirm the hypothesis that cluster formation and cluster dispersal occur concurrently during the prenatal period. Secondary myotubes continue to form until birth. At birth, the soleus muscle contains the adult number of myofibers. The first 5 days postnatally are marked by myofiber growth and maturation.