Oxidation of fluorescent glycero- and sphingophospholipids in human plasma lipoproteins: Alkenylacyl subclasses are preferred targets
Oxidation of fluorescent glycero- and sphingophospholipids in human plasma lipoproteins: Alkenylacyl subclasses are preferred targets
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DOI：
10.1016/0009-9120(96)00061-6
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发表时间：
1996-10-01
影响因子：
2.8
通讯作者：
Hermetter, A
中科院分区：
文献类型：
--
作者：
Hofer, G;Lichtenberg, D;Hermetter, A
目的：脂蛋白中多不饱和脂肪酸的氧化被认为在动脉粥样硬化形成的早期阶段起着至关重要的作用。多不饱和脂质（PUFA）被氧化，因此，脂质氧化的程度和速率取决于它们的浓度，可能还取决于它们所连接的脂质部分。设计和方法：为了确定鞘脂和甘油脂结合的脂肪酰基链的相对氧化亲合力，我们使用了相应化合物的荧光类似物，结果：在Cu ~（2+）或AAPH存在下，荧光酰基链的氧化亲和性一般取决于它们所结合的磷脂和脂蛋白。HDL中的磷脂氧化速度快于LDL或Lp（a）。缩醛磷脂比磷脂酰胆碱和鞘磷脂更容易氧化。结论：因此，HDL和缩醛磷脂可以被认为是脂质氧化的首选目标之前，大部分的多不饱和磷脂（主要是磷脂酰胆碱）在LDL是受自由基攻击。
Objectives: Oxidation of polyunsaturated fatty acids in lipoproteins is supposed to play a crucial role at the early stages of atherogenesis. The polyunsaturated lipids (PUFAs) become oxidized and, thus, the degree and rate of lipid oxidation depend on their concentration and, probably, on the lipid moiety to which they are attached.Design and methods: To determine the relative oxidation susceptibilities of sphingo- and glycerolipid-bound fatty acyl chains, we used fluorescent analogs of the respective compounds, in which one natural fatty acyl chain was replaced by fluorescent diphenylhexatriene propionic acid.Results: Oxidation susceptibilities of the fluorescent acyl chains in the presence of Cu2+ or AAPH depended, in general, on the phospholipid to which they were bound and the lipoprotein. Phospholipids were oxidized faster in HDL than in LDL or Lp(a). Plasmalogens were more susceptible to oxidation than phosphatidylcholine and sphingomyelin.Conclusion: Thus, HDL and plasmalogens may be considered as preferred targets of lipid oxidation before the bulk of polyunsaturated phospholipids (mainly phosphatidylcholine) in LDL is subject to free radical attack.