Silencing of WIPK and SIPK Mitogen-Activated Protein Kinases Reduces Tobacco mosaic virus Accumulation But Permits Systemic Viral Movement in Tobacco Possessing the N Resistance Gene
Silencing of WIPK and SIPK Mitogen-Activated Protein Kinases Reduces Tobacco mosaic virus Accumulation But Permits Systemic Viral Movement in Tobacco Possessing the N Resistance Gene
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DOI：
10.1094/mpmi-23-8-1032
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发表时间：
2010-08-01
影响因子：
3.5
通讯作者：
Ohashi, Yuko
中科院分区：
文献类型：
--
作者：
Kobayashi, Michie;Seo, Shigemi;Ohashi, Yuko
烟草花叶病毒（Tobacco mosaic virus，TMV）感染具有抗氮基因的烟草品种后，在侵染部位产生坏死性病斑，使病毒被限制在病斑内。虽然丝裂原活化蛋白激酶WIPK和SIPK与TMV抗性有关，但将它们与抗病性直接联系起来的证据还不充分。病毒增殖略有减少WIPK或SIPK沉默的植物，但在WIPK/SIPK沉默的植物，并与水杨酸（SA）的增加和茉莉酸（JA）的减少。在缺乏N基因的烟草品种中沉默WIPK和SIPK并不能抑制病毒的积累。通过表达SA降解酶的基因或通过外源施用JA来减弱病毒积累的减少。下部叶片接种导致TMV的系统传播，并在未接种的上部叶片中形成坏死病变。这些结果表明，WIPK和SIPK的功能，负调控局部抗TMV积累，部分通过调节SA和JA的积累，在N依赖的方式，但积极调节系统的抗性。
Infection of tobacco cultivars possessing the N resistance gene with Tobacco mosaic virus (TMV) results in confinement of the virus by necrotic lesions at the infection site. Although the mitogen-activated protein kinases WIPK and SIPK have been implicated in TMV resistance, evidence linking them directly to disease resistance is, as yet, insufficient. Viral multiplication was reduced slightly in WIPK- or SIPK-silenced plants but substantially in WIPK/SIPK-silenced plants, and was correlated with an increase in salicylic acid (SA) and a decrease in jasmonic acid (JA). Silencing of WIPK and SIPK in a tobacco cultivar lacking the N gene did not inhibit viral accumulation. The reduction in viral accumulation was attenuated by expressing a gene for an SA-degrading enzyme or by exogenously applying JA. Inoculation of lower leaves resulted in the systemic spread of TMV and formation of necrotic lesions in uninoculated upper leaves. These results suggested that WIPK and SIPK function to negatively regulate local resistance to TMV accumulation, partially through modulating accumulation of SA and JA in an N-dependent manner, but positively regulate systemic resistance.