Analysis of CD8+CD28- T-suppressor cells in living donor liver transplant recipients.
Analysis of CD8+CD28- T-suppressor cells in living donor liver transplant recipients.
复制标题
DOI：
--
复制
发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Yi-Xin Lin;Lan Wang;Lu-nan Yan;Pei Cai;Bo Li;T. Wen;Yong Zeng
中科院分区：
文献类型：
--
作者：
Yi-Xin Lin;Lan Wang;Lu-nan Yan;Pei Cai;Bo Li;T. Wen;Yong Zeng
背景技术人类CD8+CD28-T抑制(Ts)细胞被认为表明儿科肝肠移植受者和死亡心肾移植受者对免疫抑制的需求减少。然而，在成人对成人活体肝移植（A-A LDLT）中，可用信息很少，临床意义仍不清楚。方法采用流式细胞术检测A-A LDLT受者(n=31)、终末期肝病患者(n=24)和健康对照者(n=19)外周血中存在的CD8+CD28-Ts细胞群。同时，我们测试了受者的移植物功能和免疫抑制谷值。对 31 名移植受者的临床和随访数据进行了分析。结果与患病对照（P=0.007）和健康个体（P=0.000）相比，A-A LDLT 接受者的 CD8+CD28- Ts 细胞显着扩增。这与移植物功能、免疫抑制水平和排斥反应有关。结论 监测CD8+CD28-Ts细胞水平对于评估受者的免疫状态具有重要意义。同时，促进A-A LDLT受者体内CD8+CD28-Ts细胞的扩增也很重要，不仅可以维持良好的移植功能、减少免疫抑制剂的用量，而且可以减少排斥反应的发生。
BACKGROUND Human CD8+CD28- T-suppressor (Ts) cells have been considered to indicate a reduced need for immunosuppression in pediatric liver-intestine transplant recipients and recipients of deceased heart-kidney transplants. However, in adult-to-adult living donor liver transplantation (A-A LDLT) little information is available and the clinical significance is still unknown. METHODS Flow cytometry was used to detect the population of CD8+CD28- Ts cells present in peripheral blood in A-A LDLT recipients (n=31), patients with end-stage liver disease (n=24) and healthy controls (n=19). Meanwhile, we tested the graft function and trough levels of immunosuppression in recipients. The clinical and follow-up data of 31 transplant recipients were analyzed. RESULTS Compared with diseased controls (P=0.007) and healthy individuals (P=0.000), a notable expansion of CD8+CD28- Ts cells was found in recipients of A-A LDLT. This was associated with graft function, levels of immunosuppression and rejection episodes. CONCLUSIONS To monitor the CD8+CD28- Ts cells levels is important to evaluate the immune state of recipients. Meanwhile, it is also important to promote expansion of CD8+CD28- Ts cells in recipients of A-A LDLT, not only to sustain good graft function and decrease the dosage of immunosuppressants, but also to reduce the occurrence of rejection.