Enhanced resistance to Bacillus thuringiensis Cry1Ac toxin mediated by the activation of prophenoloxidase in a cosmopolitan pest.
Enhanced resistance to Bacillus thuringiensis Cry1Ac toxin mediated by the activation of prophenoloxidase in a cosmopolitan pest.
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DOI：
10.1016/j.ijbiomac.2023.124678
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发表时间：
2023-05
影响因子：
8.2
通讯作者：
Zeyun Li;Lei Xiong;Jingge Li;Shuyuan Yao;Yi Dong;Yongbin Li;Xuanhao Chen;Min Ye;Yangrui Zhang;Xuefeng Xie;M. You;Z. Yuchi;Yuanyuan Liu;Shijun You
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文献类型：
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作者：
Zeyun Li;Lei Xiong;Jingge Li;Shuyuan Yao;Yi Dong;Yongbin Li;Xuanhao Chen;Min Ye;Yangrui Zhang;Xuefeng Xie;M. You;Z. Yuchi;Yuanyuan Liu;Shijun You
小菜蛾在漫长的进化过程中进化出了对苏云金杆菌cry1ac毒素的抗性。增强的免疫反应是昆虫对多种杀虫剂产生抗性的重要因素，而免疫蛋白苯酚氧化酶（PO）是否参与了昆虫对Cry1Ac毒素的抗性。xylostellaremains不清楚。时空表达模式显示，cry1s1000抗性品系的pxppo1和pxppo2在卵、4龄、头部和血淋巴中的表达高于g88敏感品系。PO活性分析结果表明，Cry1Ac毒素处理后的PO活性比处理前提高了约3倍。此外，敲除pxppo1和pxppo2显著增加了对Cry1Ac毒素的敏感性。在cry1s1000抗性菌株中，敲低ppo负调控因子clip - sph2导致pxppo1和pxppo2表达增加，并增加了Cry1Ac敏感性，进一步支持了这些发现。槲皮素的协同作用表明，与对照组相比，幼虫存活率从100%下降到< 20%。本研究将为白粉病抗性机制相关免疫相关基因（PO）的分析及害虫防治提供理论依据。蛾。
Plutella xylostellahas evolved resistance toBacillus thuringiensisCry1Ac toxin over a long evolutionary period. Enhanced immune response is an important factor in insect resistance to a variety of insecticides, and whether phenoloxidase (PO), an immune protein, is involved in resistance to Cry1Ac toxin inP. xylostellaremains unclear. Here, spatial and temporal expression patterns showed that prophenoloxidase (PxPPO1andPxPPO2) in the Cry1S1000-resistant strain was more highly expressed in eggs, 4th instar, head, and hemolymph than those in G88-susceptible strain. The results of PO activity analysis showed that after treatment with Cry1Ac toxin PO activity was about 3 times higher than that before treatment. Furthermore, knockout ofPxPPO1andPxPPO2significantly increased the susceptibility to Cry1Ac toxin. These findings were further supported by the knockdown ofClip-SPH2, a negative regulator of PO, which resulted in increasedPxPPO1andPxPPO2expression and Cry1Ac susceptibility in the Cry1S1000-resistant strain. Finally, the synergistic effect of quercetin showed that larval survival decreased from 100 % to <20 % compared to the control group. This study will provide a theoretical basis for the analysis of immune-related genes (PO) genes involved in the resistance mechanism and pest control ofP. xylostella.