Bovine-Like Coronaviruses Isolated from Four Species of Captive Wild Ruminants Are Homologous to Bovine Coronaviruses, Based on Complete Genomic Sequences
Bovine-Like Coronaviruses Isolated from Four Species of Captive Wild Ruminants Are Homologous to Bovine Coronaviruses, Based on Complete Genomic Sequences
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DOI：
10.1128/jvi.01586-08
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发表时间：
2008-12-15
影响因子：
5.4
通讯作者：
Saif, Linda J.
中科院分区：
文献类型：
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作者：
Alekseev, Konstantin P.;Vlasova, Anastasia N.;Saif, Linda J.
我们对四种冠状病毒（cov）的全长基因组进行了测序和分析，每种冠状病毒（cov）都来自俄亥俄州一种不同的野生反刍动物物种：鹿（Cervus unicolor）、水羚（Kobus ellipsiprymnus）、黑羚羊（Hippotragus niger）和白尾鹿（Odocoileus virginianus）。在1993年和1994年冬季痢疾暴发和散发性腹泻病例中采集了鹿、水羚和白尾鹿的粪便样本（H. Tsunemitsu， Z. R. el-Kanawati， D. R. Smith， H. H. Reed， L. J. Saif， J. clinin）。微生物学杂志。33:364 -3269,1995)。2003年，在美国俄亥俄州野生动物栖息地分离出一种长颈鹿类牛冠状病毒（giicov），并采集了一种貂羚羊粪便样本（M. Hasoksuz， K. Alekseev， A. Vlasova, Zhang X. D. Spiro， R. Halpin， S. Wang， E. Ghedin， L. J. Saif， J.病毒学报，81:481 - 490,2007）。对于两种冠状病毒（鹿和水牛），还对细胞培养适应株和非生物小牛传代株的完整基因组进行了测序和分析。在系统发育上，野生反刍动物冠状病毒属于2a群冠状病毒，与最近的牛冠状病毒（BCoV）菌株关系最密切。野生反刍动物毒株与新近分离的BCoV毒株（1998年分离的BCoV- lun和BCoV- ent）的高核苷酸同源性（99.4 ~ 99.6%）进一步证实了亲缘关系。圈养野生反刍动物的冠状病毒与BCoV毒株的遗传比较分析表明，没有特定的基因组标记可以区分圈养野生反刍动物的牛毒株和牛样冠状病毒；此外，没有发现特定的遗传标记来确定细胞培养或小牛传代菌株或菌株的宿主来源。这项研究的结果证实了先前关于牛和野生反刍动物冠状病毒之间生物学和抗原相似性的报道，并表明牛可能是感染圈养野生反刍动物的冠状病毒的宿主，反之亦然，这些冠状病毒可能代表一种祖先冠状病毒的宿主范围变体。
We sequenced and analyzed the full-length genomes of four coronaviruses (CoVs), each from a distinct wild-ruminant species in Ohio: sambar deer (Cervus unicolor), a waterbuck (Kobus ellipsiprymnus), a sable antelope (Hippotragus niger), and a white-tailed deer (Odocoileus virginianus). The fecal samples from the sambar deer, the waterbuck, and the white-tailed deer were collected during winter dysentery outbreaks and sporadic diarrhea cases in 1993 and 1994 (H. Tsunemitsu, Z. R. el-Kanawati, D. R. Smith, H. H. Reed, and L. J. Saif, J. Clin. Microbiol. 33: 3264-3269, 1995). A fecal sample from a sable antelope was collected in 2003 from an Ohio wild-animal habitat during the same outbreak when a bovine-like CoV from a giraffe (GiCoV) was isolated (M. Hasoksuz, K. Alekseev, A. Vlasova, X. Zhang, D. Spiro, R. Halpin, S. Wang, E. Ghedin, and L. J. Saif, J. Virol. 81: 4981-4990, 2007). For two of the CoVs (sambar deer and waterbuck), complete genomes from both the cell culture-adapted and gnotobiotic-calf-passaged strains were also sequenced and analyzed. Phylogenetically, wild-ruminant CoVs belong to group 2a CoVs, with the closest relatedness to recent bovine CoV (BCoV) strains. High nucleotide identities (99.4 to 99.6%) among the wild-ruminant strains and recent BCoV strains (BCoV-LUN and BCoV-ENT, isolated in 1998) further confirm the close relatedness. Comparative genetic analysis of CoVs of captive wild ruminants with BCoV strains suggests that no specific genomic markers are present that allow discrimination between the bovine strains and bovine-like CoVs from captive wild ruminants; furthermore, no specific genetic markers were identified that defined cell cultured or calf-passaged strains or the host origin of strains. The results of this study confirm prior reports of biologic and antigenic similarities between bovine and wild-ruminant CoVs and suggest that cattle may be reservoirs for CoVs that infect captive wild ruminants or vice versa and that these CoVs may represent host range variants of an ancestral CoV.