Retroposition of processed pseudogenes: the impact of RNA stability and translational control
Retroposition of processed pseudogenes: the impact of RNA stability and translational control
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DOI：
10.1016/j.tig.2005.11.005
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发表时间：
2006-02-01
影响因子：
11.4
通讯作者：
Jurka, J
中科院分区：
文献类型：
--
作者：
Pavlicek, A;Gentles, AJ;Jurka, J
人类加工假基因是细胞RNA的拷贝，通过L1(LINE1)非LTR反转录转座子的酶机制逆转录并插入到核基因组中。虽然人们普遍认为胚系表达对细胞mRNAs的可遗传逆位至关重要，但关于RNA稳定性、mRNA质量控制和翻译区分化对加工假基因逆位的影响尚不清楚。我们发现，经常逆转录的人类mRNAs来自稳定的转录本，具有翻译能力的功能阅读框架，可以抵抗无义介导的RNA衰退。它们优先翻译在游离的细胞质核糖体上，并编码可溶性蛋白。我们的结果表明，mRNAs和L1蛋白之间的相互作用似乎发生在游离的细胞质核糖体上。
Human processed pseudogenes are copies of cellular RNAs reverse transcribed and inserted into the nuclear genome by the enzymatic machinery of L1 (LINE1) non-LTR retrotransposons. Although it is generally accepted that germline expression is crucial for the heritable retroposition of cellular mRNAs, little is known about the influences of RNA stability, mRNA quality control and compartmentalization of translation on the retroposition of processed pseudogenes. We found that frequently retroposed human mRNAs are derived from stable transcripts with translation-competent functional reading frames that are resistant to nonsense-mediated RNA decay. They are preferentially translated on free cytoplasmic ribosomes and encode soluble proteins. Our results indicate that interactions between mRNAs and L1 proteins seem to occur at free cytoplasmic ribosomes.