Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench)
Preliminary characterization and anti-hyperglycemic activity of a pectic polysaccharide from okra (Abelmoschus esculentus (L.) Moench)
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DOI：
10.1016/j.jff.2017.12.028
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发表时间：
2018-02
影响因子：
5.6
通讯作者：
Tao Zhang;Jialin Xiang;Guo-dong Zheng;Ren‐Jie Yan;Xun Min
中科院分区：
文献类型：
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作者：
Tao Zhang;Jialin Xiang;Guo-dong Zheng;Ren‐Jie Yan;Xun Min
秋葵在中国被广泛用作蔬菜、食品配料和传统药物，用途广泛。秋葵的主要健康特性被认为是源于其多糖含量高。本研究通过DEAE-cellulose和Sepharose CL-6B柱从秋葵中提取分离纯化了一种均质果胶多糖WOP-2，其分子量估计为580 kDa。单糖组成分别为Rha（21.4%）、GalA（34.9%）、Gal（29.6%）、GlcA（4.5%）、Glc（5.9%）和Ara（3.7%）。结合核磁共振分析，WOP-2被证实是鼠李糖半乳聚糖I骨架，其ii型阿拉伯半乳聚糖侧链部分取代于Rhap的O-4。WOP-2可能通过抑制脂质过氧化链反应对stz诱导的糖尿病小鼠表现出显著的抗高血糖活性。总之，这些结果为秋葵多糖的结构-活性关系提供了新的认识，并为基于多糖的糖尿病治疗的发展提供了动力。
Okra is widely used as vegetable, food ingredient, and traditional medicine in China for various applications. The main healthy properties are accepted to originate from the high polysaccharide content of okra. In this study, a homogenous pectic polysaccharide WOP-2 was extracted, isolated, and purified from okra by DEAE-cellulose and Sepharose CL-6B column, and its molecular weight was estimated to be 580 kDa. Monosaccharide composition was analyzed to be Rha (21.4%), GalA (34.9%), Gal (29.6%), GlcA (4.5%), Glc (5.9%), and Ara (3.7%) by HPLC with PMP derivatization. Combined with NMR analysis, WOP-2 was elucidated to be a rhamnogalacturonan I backbone with type-II arabinogalactan side-chains substituted partly at O-4 of Rhap. WOP-2 exhibits significant anti-hyperglycemic activity on STZ-induced diabetic mice by inhibiting the lipid peroxidation chain reaction probably. Overall, these results provide new insight into structure–activity relationships of polysaccharide from okra and provide impetus towards the development of polysaccharide-based therapeutics on diabetes.