Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice.
Phosphorylation-dependent interaction of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (YWHA) with PADI6 following oocyte maturation in mice.
复制标题
小鼠卵母细胞成熟后酪氨酸 3-单加氧酶/色氨酸 5-单加氧酶激活蛋白 (YWHA) 与 PADI6 的磷酸化依赖性相互作用。
DOI：
10.1095/biolreprod.108.069328
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发表时间：
2008
影响因子：
3.6
通讯作者：
Kline,Douglas
中科院分区：
文献类型：
--
作者：
Snow,AlanJ;Puri,Pawan;Acker-Palmer,Amparo;Bouwmeester,Tewis;Vijayaraghavan,Srinivasan;Kline,Douglas
酪氨酸3-单加氧酶/色氨酸5-单加氧酶激活蛋白家族（YWHA;也称为14-3-3）中的蛋白质参与许多细胞内过程的调节。我们研究了YWHA与肽基精氨酸脱亚胺酶VI型（PADI 6）的相互作用，PADI 6是哺乳动物卵母细胞、卵子和早期胚胎中丰富的蛋白质。肽基精氨酸脱亚胺酶催化肽基精氨酸翻译后修饰为瓜氨酸。PADI 6与卵母细胞胞质片层相关，PADI 6缺陷小鼠由于发育中断超过两细胞阶段而不育。我们发现PADI 6在卵母细胞成熟过程中磷酸化发生了显著的发育变化。这种磷酸化的变化与成熟卵子中PADI 6与YWHA的相互作用有关。重组谷胱甘肽S-转移酶YWHA下拉实验和转基因串联亲和纯化与液相色谱-质谱证明YWHA和PADI 6在成熟的鸡蛋之间的结合相互作用。YWHA蛋白调节或补充涉及磷酸化依赖性开关或蛋白质修饰的细胞内事件。这些结果表明，磷酸化和/或YWHA结合可以作为细胞内PADI 6调节的手段。
Proteins in the tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein family (YWHA; also known as 14-3-3) are involved in the regulation of many intracellular processes. We have examined the interaction of YWHA with peptidylarginine deiminase type VI (PADI6), an abundant protein in mammalian oocytes, eggs, and early embryos. Peptidylarginine deiminases catalyze the posttranslational modification of peptidylarginine to citrulline. PADI6 is associated with oocyte cytoplasmic sheets, and PADI6-deficient mice are infertile because of disruption of development beyond the two-cell stage. We found that PADI6 undergoes a dramatic developmental change in phosphorylation during oocyte maturation. This change in phosphorylation is linked to an interaction of PADI6 with YWHA in the mature egg. Recombinant glutathioneS-transferase YWHA pull-down experiments and transgenic tandem affinity purification with liquid chromatography-mass spectrometry demonstrate a binding interaction between YWHA and PADI6 in mature eggs. YWHA proteins modulate or complement intracellular events involving phosphorylation-dependent switching or protein modification. These results indicate that phosphorylation and/or YWHA binding may serve as a means of intracellular PADI6 regulation.