Longitudinal Peripheral Blood Lymphocyte Subsets Correlate with Decreased Disease Activity in Juvenile Dermatomyositis
Longitudinal Peripheral Blood Lymphocyte Subsets Correlate with Decreased Disease Activity in Juvenile Dermatomyositis
复制标题
纵向外周血淋巴细胞亚群与幼年皮肌炎疾病活动性降低有关
DOI：
10.3899/jrheum.121031
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发表时间：
2013-07-01
影响因子：
3.9
通讯作者：
Reed, Ann M.
中科院分区：
文献类型：
--
作者：
Ernste, Floranne C.;Crowson, Cynthia S.;Reed, Ann M.
Objective.目的探讨幼年型皮肌炎（JDM）患者的临床特征及外周血淋巴细胞（PBL）亚群的变化。2007年至2011年期间，在马约诊所罗切斯特采集了24例JDM患者的外周血单核细胞。使用荧光激活细胞分选和流式细胞术对这些进行分析。通过连续2次访视中收集的视觉模拟量表（VAS）确定临床疾病活动度，并与PBL亚组相关。CD 3 + CD 69 + T细胞的变化与总体VAS评分的变化相关。HLA-DR-CD 11 c+髓样树突状细胞的变化也与肌外VAS评分的变化相关。随着肌肉和整体VAS评分下降，HLA-DR-CD 11 c+细胞水平有下降的趋势，但这些并没有达到显着性。HLA-DR-CD 123+浆细胞样树突状细胞的变化与肌肉VAS评分的变化呈负相关。虽然没有统计学意义，但CD 3-CD 16-CD 56+自然杀伤（NK）细胞和HLA-DR-CD 86+髓样树突状细胞水平的降低以及CD 16 + CD 56- NK细胞水平的升高与VAS评分降低相关。CD 3 + CD 69 + T细胞、HLA-DR-CD 11 c+髓样树突状细胞和HLA-DR-CD 123+浆细胞样树突状细胞的变化与JDM的临床病程改善相关，可用作疾病活动性的标志物，但研究结果需要在更大的独立队列中进行验证。大多数PBL亚群之间缺乏显著差异表明，淋巴细胞表型可能很难与JDM的疾病活动性明确相关。
Objective. To determine the clinical characteristics and subsets of peripheral blood lymphocytes (PBL), which correlate with decreased disease activity in patients with juvenile dermatomyositis (JDM).Methods. Peripheral blood mononuclear cells from 24 patients with JDM were collected at Mayo Clinic Rochester between 2007 and 2011. These were analyzed using fluorescence-activated cell sorting and flow cytometry. Clinical disease activity was determined by visual analog scales (VAS) collected in 2 consecutive visits and correlated with PBL subsets.Results. The change in CD3+CD69+ T cells correlated with the change in global VAS scores. The change in HLA-DR- CD11c+ myeloid dendritic cells also correlated with the change in extramuscular VAS scores. There were trends toward decreased levels of HLA-DR- CD11c+ cells with decreased muscle and global VAS scores, but these did not reach significance. The change in HLA-DR- CD123+ plasmacytoid dendritic cells negatively correlated with the change in muscle VAS scores. Although not statistically significant, decreased levels of CD3-CD16- CD56+ natural killer (NK) cells and HLA-DR- CD86+ myeloid dendritic cells, and increased levels of CD16+CD56- NK cells, correlated with decreased VAS scores.Conclusion. Changes in CD3+CD69+ T cells, HLA-DR- CD11c+ myeloid dendritic cells, and HLA-DR- CD123+ plasmacytoid dendritic cells are associated with improved clinical course in JDM and could be used as markers for disease activity, but findings need to be verified in a larger; independent cohort. Lack of significant differences among most of our PBL subsets suggests that lymphocyte phenotyping may be difficult to definitively correlate with disease activity in JDM.