Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes
Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes
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DOI：
10.1038/sj.cr.7290170
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发表时间：
2003-08-01
期刊：
影响因子：
44.1
通讯作者：
Sheng, HZ
中科院分区：
文献类型：
--
作者：
Chen, Y;He, ZX;Sheng, HZ
为了解决免疫不相容性的问题，核移植已经被设想为产生用于人自体移植的细胞或组织的手段。在这里，我们通过将人的体细胞核转移到兔的卵母细胞中来获得胚胎干细胞。在5岁、42岁、52岁和60岁的核供体中，从融合核移植中发育的囊胚数量相当，随后从四个年龄组中的每一个中获得核移植（NT）胚胎干细胞（ntES细胞）。这些结果表明，人的体细胞核可以形成ntES细胞的年龄无关的供体。基于核型、同源性、原位杂交、PCR和免疫细胞化学，衍生的ntES细胞是人类的，其中探针可区分不同物种。ntES细胞在未分化状态下保持持续生长的能力，并形成胚状体，其在进一步诱导时产生细胞类型，如神经元和肌肉，以及表达代表所有三个胚层的标志物的混合细胞群。因此，通过NT至兔卵从人体细胞衍生的ntES细胞保留了与常规人ES细胞相似的表型，包括经历多谱系细胞分化的能力。
To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.