The lipid composition of drusen, Bruch's membrane, and sclera by hot stage polarizing light microscopy.
The lipid composition of drusen, Bruch's membrane, and sclera by hot stage polarizing light microscopy.
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发表时间：
2001-06
影响因子：
4.4
通讯作者：
R. Haimovici;Donald L. Gantz;S. Rumelt;T. Freddo;Donald Small
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文献类型：
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作者：
R. Haimovici;Donald L. Gantz;S. Rumelt;T. Freddo;Donald Small
目的 使用热台偏光显微镜 (HSPM) 原位检测和鉴定老化人眼中玻璃膜疣、弥漫性布鲁赫膜沉积物和巩膜的脂质成分，该方法可以根据加热和冷却过程中双折射监测的液晶形态和熔化温度来定性确定组织学切片中的脂质亚型。方法 将 17 名患者的黄斑中心和周边全层纽扣固定在 5% 钙缓冲福尔马林中。冰冻切片用油红O或苏丹黑染色或通过HSPM分析。结果 在弥漫性布鲁赫膜沉积物、玻璃膜疣和巩膜中发现了具有胆固醇酯熔化特性的双折射各向异性液滴（“马耳他十字”）。冷却时从晶体熔化为油而没有形成任何马耳他十字的沉积物存在于巩膜中，并且与富含甘油三酯的沉积物一致。在一只老化的眼睛中发现了具有与磷脂一致的光学特性的沉积物。年轻捐赠者的眼睛没有显示出这些变化。结论 HSPM 是一种评估老化眼睛脂质沉积性质的有价值的技术。需要进一步的研究来确定患有年龄相关性黄斑变性的眼睛是否也存在类似的变化。
PURPOSE To detect and identify, in situ, the lipid composition of drusen, diffuse Bruch's membrane deposits, and sclera in aging human eyes using hot-stage polarizing microscopy (HSPM), a method that allows qualitative determination of lipid subtypes within histologic sections based on morphology and melting temperatures of liquid crystals as monitored by birefringence during heating and cooling. METHODS Full-thickness buttons of the central macula and the periphery of human eyes from 17 patients were fixed in 5% calcium-buffered formalin. Frozen sections were stained with oil red O or Sudan black or were analyzed by HSPM. RESULTS Birefringent anisotropic droplets ("maltese crosses") with melting characteristics of cholesterol esters were identified within diffuse Bruch's membrane deposits, drusen, and sclera. Deposits that melted from crystal to oil without any maltese cross formation when cooled were present in the sclera and are consistent with triglyceride-rich deposits. Deposits with optical properties consistent with phospholipids were identified in a single aged eye. Eyes from young donors did not show these changes. CONCLUSIONS HSPM is a valuable technique for evaluating the nature of lipid deposits in aging eyes. Further studies are warranted to determine whether similar changes are also present in eyes with age-related macular degeneration.