Graphene in Mice: Ultrahigh In Vivo Tumor Uptake and Efficient Photothermal Therapy
Graphene in Mice: Ultrahigh In Vivo Tumor Uptake and Efficient Photothermal Therapy
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DOI：
10.1021/nl100996u
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发表时间：
2010-09-01
期刊：
影响因子：
10.8
通讯作者：
Liu, Zhuang
中科院分区：
文献类型：
--
作者：
Yang, Kai;Zhang, Shuai;Liu, Zhuang
虽然近年来碳纳米管的生物医学应用得到了广泛的研究，但其姊妹石墨烯在生物医学中的应用却很少。在这项工作中，我们第一次研究了纳米石墨烯片（NGS）与聚乙二醇（PEG）涂层的体内行为的荧光标记方法。体内荧光成像揭示了在几种异种移植肿瘤小鼠模型中NGS的令人惊讶的高肿瘤摄取。与聚乙二醇化碳纳米管不同，聚乙二醇化NGS显示出几种有趣的体内行为，包括高效的肿瘤被动靶向和在网状内皮系统中相对较低的保留。然后，我们利用NGS在近红外（NIR）区域的强光学吸收进行体内光热治疗，在静脉内施用NGS和低功率NIR激光照射肿瘤后实现超高效的肿瘤消融。此外，在我们的初步毒性研究中，通过组织学、血液化学和全血组分析，注射小鼠未观察到PEG化NGS的明显副作用。虽然需要更多的努力来进一步了解这种新型纳米材料的体内行为和长期毒理学，但我们的工作是首次成功地使用碳纳米材料通过静脉注射进行有效的体内光热治疗，并表明石墨烯在生物医学应用中的巨大前景，例如癌症治疗。
Although biomedical applications of carbon nanotubes have been intensively studied in recent years, its sister, graphene, has been rarely explored in biomedicine. In this work, for the first time we study the in vivo behaviors of nanographene sheets (NGS) with polyethylene glycol (PEG) coating by a fluorescent labeling method. In vivo fluorescence imaging reveals surprisingly high tumor uptake of NGS in several xenograft tumor mouse models. Distinctive from PEGylated carbon nanotubes, PEGylated NGS shows several interesting in vivo behaviors including highly efficient tumor passive targeting and relatively low retention in reticuloendothelial systems. We then utilize the strong optical absorbance of NGS in the near-infrared (NIR) region for in vivo photothermal therapy, achieving ultraefficient tumor ablation after intravenous administration of NGS and low-power NIR laser irradiation on the tumor. Furthermore, no obvious side effect of PEGylated NGS is noted for the injected mice by histology, blood chemistry, and complete blood panel analysis in our pilot toxicity study. Although a lot more efforts are required to further understand the in vivo behaviors and the long-term toxicology of this new type of nanomaterials, our work is the first success of using carbon nanomaterials for efficient in vivo photothermal therapy by intravenous administration and suggests the great promise of graphene in biomedical applications, such as cancer treatment.