Distribution of the voltage-gated sodium channel NaV1.7 in the rat:: Expression in the autonomic and endocrine systems
Distribution of the voltage-gated sodium channel NaV1.7 in the rat:: Expression in the autonomic and endocrine systems
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DOI：
10.1002/cne.21484
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发表时间：
2007-10-20
影响因子：
2.5
通讯作者：
O'Donnell, Dajan
中科院分区：
文献类型：
--
作者：
Morinville, Anne;Fundin, Bengt;O'Donnell, Dajan
一般认为，电压门控的河豚毒素敏感钠通道Na(v)1.7在周围神经节中选择性表达。然而，Nav1.7的整体缺失会导致小鼠出生后不久死亡(Nassar et al. [2004] Proc. Natl。学会科学。u.s. 101:12706-12711)，这表明该离子通道可能更广泛地表达。为了更好地了解该离子通道的潜在生理功能，我们采用原位杂交和免疫组织化学方法检测了Nav1.7在大鼠中的表达。正如预期的那样，mRNA的最高表达水平在外周神经节中被发现，蛋白质在这些神经节细胞和中枢神经系统中这些神经元的突起上表达。重要的是，我们发现Na(v)1.7存在于离散的大鼠大脑区域，其独特的分布模式暗示了中枢参与内分泌和自主神经系统以及镇痛。此外，在垂体和肾上腺中检测到Nav1.7的表达。这些结果表明，Nav1.7不仅参与感觉信息的加工，还参与自主神经和内分泌系统的调节；更具体地说，它可能涉及诸如体液稳态和心血管控制等重要功能。
It is generally accepted that the voltage-gated, tetrodotoxin-sensitive sodium channel, Na(v)1.7, is selectively expressed in peripheral ganglia. However, global deletion in mice of Nav1.7 leads to death shortly after birth (Nassar et al. [2004] Proc. Natl. Acad. Sci. U. S. A. 101:12706-12711), suggesting that this ion channel might be more widely expressed. To understand better the potential physiological function of this ion channel, we examined Nav1.7 expression in the rat by in situ hybridization and immunohistochemistry. As expected, highest mRNA expression levels are found in peripheral ganglia, and the protein is expressed within these ganglion cells and on the projections of these neurons in the central nervous system. Importantly, we found that Na(v)1.7 is present in discrete rat brain regions, and the unique distribution pattern implies a central involvement in endocrine and autonomic systems as well as analgesia. In addition, Nav1.7 expression was detected in the pituitary and adrenal glands. These results indicate that Nav1.7 is not only involved in the processing of sensory information but also participates in the regulation of autonomic and endocrine systems; more specifically, it could be implicated in such vital functions as fluid homeostasis and cardiovascular control.