Ulcerative colitis and Crohn's disease: distinctive gene expression profiles and navel susceptibility candidate genes
Ulcerative colitis and Crohn's disease: distinctive gene expression profiles and navel susceptibility candidate genes
复制标题
DOI：
10.1093/hmg/10.5.445
复制
发表时间：
2001-03-01
影响因子：
3.5
通讯作者：
Chakravarti, S
中科院分区：
文献类型：
--
作者：
Lawrance, IC;Fiocchi, C;Chakravarti, S
为了阐明两种形式的炎症性肠病（IBD）、溃疡性结肠炎（UC）和克罗恩病（CD）背后的生物失调，我们使用DNA微阵列检查了发炎结肠组织的整体基因表达谱。我们的结果发现了几个表达改变的基因，这些基因以前与 IBD 没有关联。除了各种细胞因子和趋化因子基因的预期上调外，新的免疫功能相关基因（例如 IGHG3、IGLL2 和 CD74）、炎症相关的脂质运载蛋白 HNL 和 NGAL 以及增殖相关的 GRO 基因在 UC 中过度表达。某些癌症相关基因，如 DD96、DRAL 和 MXI1 仅在 UC 中差异表达。在 UC 和 CD 中过度表达的其他基因包括 REG 基因家族和钙结合 S100 蛋白基因 S100A9 和 S100P。天然抗菌防御素 DEFA5 和 DEFA6 基因在 CD 中尤其过度表达。总体而言，170 个基因的表达谱存在显着差异，将 UC 和 CD 确定为不同的分子实体。失调基因的基因组图谱位置可能会识别出 UC 和 CD 遗传易感性的新候选基因。
To elucidate the biological dysregulation underlying two forms of inflammatory bowel disease (IBD), ulcerative colitis (UC) and Crohn's disease (CD), we examined global gene expression profiles of inflamed colonic tissue using DNA microarrays. Our results identified several genes with altered expression not previously linked to IBD. In addition to the expected upregulation of various cytokine and chemokine genes, novel immune function-related genes such as IGHG3 IGLL2 and CD74, inflammation-related lipocalins HNL and NGAL, and proliferation-related GRO genes were over-expressed in UC. Certain cancer-related genes such as DD96, DRAL and MXI1 were differentially expressed only in UC. Other genes over-expressed in both UC and CD included the REG gene family and the calcium-binding S100 protein genes S100A9 and S100P. The natural antimicrobial defensin DEFA5 and DEFA6 genes were particularly over-expressed in CD. Overall, significant differences in the expression profiles of 170 genes identified UC and CD as distinct molecular entities. The genomic map locations of the dysregulated genes may identify novel candidates for UC and CD genetic susceptibility.