Phytochemical and underlying mechanism of Mikania micrantha Kunth on antibiotic resistance genes, and pathogenic microbes during chicken manure composting.
Phytochemical and underlying mechanism of Mikania micrantha Kunth on antibiotic resistance genes, and pathogenic microbes during chicken manure composting.
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DOI：
10.1016/j.biortech.2022.128241
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发表时间：
2022-11
影响因子：
11.4
通讯作者：
Yousif Abdelrahman Yousif Abdellah-Yousif-Abdelrahman-Yousif-Abdellah-2159139355;Yuan Luo;Shan-Shan Sun-Shan;Xi Yang;H. Ji;Rui-Long Wang
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文献类型：
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作者：
Yousif Abdelrahman Yousif Abdellah-Yousif-Abdelrahman-Yousif-Abdellah-2159139355;Yuan Luo;Shan-Shan Sun-Shan;Xi Yang;H. Ji;Rui-Long Wang
鸡粪是抗生素抗性基因（ARGs）和病原微生物的来源，薇甘菊（Mikania micranthaKunth，MM）是一种含有植物化学物质的外来入侵植物。为了探索其对ARG和病原体-宿主相互作用（PHIs）的影响，将MM添加到堆肥混合物中。结果表明，与对照相比，MM显著提高了堆肥中植物化学成分的含量，尤其是芪类和二芳基庚烷类化合物的含量（4.87%）和泛醌类化合物的含量（2.66%）。除此之外，还观察到ARGs显著减少，其中rpoB 2、RbpA、FosB 1、vatC和vatB从T堆肥中去除。在T堆肥中，PHIs显著降低，柑橘黄单胞菌、肺炎链球菌、禾谷镰刀菌、霍乱弧菌和野油菜黄单胞菌的生长受到抑制。多变量分析表明，温度和pH值在ARGs和PHIs下降中起着重要作用。因此，本研究相当推荐MM作为一种有前途的堆肥添加剂，其对病原微生物和ARG的抗菌潜力。
Chicken manure is a source of antibiotic resistance genes (ARGs) and pathogenic microbes.Mikania micranthaKunth (MM) is an invasive plant containing phytochemicals as antimicrobial agents. To explore its impacts on ARGs and pathogen-host interactions (PHIs), MM was added to composting mixtures. The findings indicated that compared with control (CK), MM significantly improved the phytochemical abundances, particularly stilbenoids and diarylheptanoids (4.87%), and ubiquinones (2.66%) in the treatment (T) compost. Besides, significant ARGs reduction was noted, whererpoB2,RbpA,FosB1,vatC, andvatBwere removed from T compost. PHIs significantly declined in T compost, where the growth ofXanthomonas citri,Streptococcus pneumoniae,Fusarium graminearum,Vibrio cholerae, andXanthomonas campestriswere inhibited. Multiple variable analyses demonstrated that temperature and pH revealed a significant role in ARGs and PHIs decline. Accordingly, this study considerably recommends MM as a promising compost additive in terms of its antimicrobial potential toward pathogenic microbes and ARGs.