Dynamic and scalable DNA-based information storage
Dynamic and scalable DNA-based information storage
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DOI：
10.1038/s41467-020-16797-2
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发表时间：
2020-06-12
影响因子：
16.6
通讯作者：
Keung, Albert J.
中科院分区：
文献类型：
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作者：
Lin, Kevin N.;Volkel, Kevin;Keung, Albert J.
信息存储系统的物理体系结构通常决定了信息如何编码、数据库如何组织以及文件如何访问。在这里，我们展示了一个由T7启动子和单链悬空结构域（ss-dsDNA）组成的简单架构，可以以强大的功能和优势解锁基于dna的动态信息存储。悬垂提供了一个物理地址，用于访问特定的DNA链以及实现一系列存储文件操作。它通过扩展可编码序列空间来提高理论存储密度和容量，并简化正交文件地址集设计的计算负担。同时，T7启动子通过从DNA中转录信息而不破坏DNA，从而实现可重复的信息访问。此外，围绕T7启动子的饱和诱变和对环境条件的系统分析揭示了可用于优化信息获取的设计标准。这种简单但功能强大的ss-dsDNA体系结构为具有多种功能的信息存储奠定了基础。信息存储的物理架构决定了数据如何编码、组织和访问。在这里，作者使用带有单链悬垂的DNA作为物理地址来访问特定的数据，并以可扩展和可重用的方式进行存储文件操作。
The physical architectures of information storage systems often dictate how information is encoded, databases are organized, and files are accessed. Here we show that a simple architecture comprised of a T7 promoter and a single-stranded overhang domain (ss-dsDNA), can unlock dynamic DNA-based information storage with powerful capabilities and advantages. The overhang provides a physical address for accessing specific DNA strands as well as implementing a range of in-storage file operations. It increases theoretical storage densities and capacities by expanding the encodable sequence space and simplifies the computational burden in designing sets of orthogonal file addresses. Meanwhile, the T7 promoter enables repeatable information access by transcribing information from DNA without destroying it. Furthermore, saturation mutagenesis around the T7 promoter and systematic analyses of environmental conditions reveal design criteria that can be used to optimize information access. This simple but powerful ss-dsDNA architecture lays the foundation for information storage with versatile capabilities. The physical architectures of information storage dictate how data is encoded, organised and accessed. Here the authors use DNA with a single-strand overhang as a physical address to access specific data and do in-storage file operations in a scalable and reusuable manner.