IDENTIFICATION OF MALARIA SPECIES BY ELISA IN SPOROZOITE AND OOCYST INFECTED ANOPHELES FROM WESTERN KENYA
IDENTIFICATION OF MALARIA SPECIES BY ELISA IN SPOROZOITE AND OOCYST INFECTED ANOPHELES FROM WESTERN KENYA
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DOI：
10.4269/ajtmh.1988.39.323
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发表时间：
1988-10-01
影响因子：
3.3
通讯作者：
BRANDLINGBENNETT, AD
中科院分区：
文献类型：
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作者：
BEIER, MS;SCHWARTZ, IK;BRANDLINGBENNETT, AD
采用恶性疟原虫、三日疟原虫和卵圆疟原虫环子孢子（CS）抗原的酶联免疫吸附试验（ELISA）来鉴定冈比亚按蚊解剖检测到的子孢子和卵囊感染的种类和一个.在肯尼亚西部采集的funestus。ELISA鉴定了1，113例唾液腺感染中的92.5%;疟原虫种感染包括79.4%的恶性疟原虫、3.2%的三日疟原虫、1.7%的卵形疟原虫，在15.7%的按蚊中检测到2种或2种以上疟原虫，其中鉴定了寄生虫种。在解剖中观察到的子孢子数量越多，鉴定的可能性就越大，从唾液腺中具有1-10个子孢子的蚊子的65% ELISA阳性增加到具有超过1，000个子孢子的蚊子的96%。ELISA检测CS抗原的294按蚊，解剖有卵囊，但未感染的唾液腺中的66%。在112只在唾液腺中检测到单一疟原虫的按蚊中，29只（25.9%）在中肠中检测到1种或多种其他疟原虫，表明多重感染的可能性很高。类似比例的疟原虫物种被发现在一个。冈比亚有限公司和一个. funestus。
Enzyme-linked immunosorbent assays (ELISAs) for the circumsporozoite (CS) antigens of Plasmodium falciparum, P. malariae, and P. ovale were used to identify species of sporozoite and oocyst infections detected by dissection in Anopheles gambiae s.l. and An. funestus collected in western Kenya. ELISAs identified 92.5% of 1,113 salivary gland infections; Plasmodium species infections included 79.4% P. falciparum, 3.2% P. malariae, 1.7% P. ovale and 2 or more Plasmodium species were detected in 15.7% of the Anopheles in which the species of parasite was identified. Identification was more likely with greater numbers of sporozoites observed in dissections, increasing from 65% ELISA positivity in mosquitoes with 1-10 sporozoites in their salivary glands to 96% in mosquitoes with over 1,000 sporozoites. ELISAs detected CS antigen in 66% of 294 Anopheles that by dissection had oocysts but uninfected salivary glands. Of 112 Anopheles with a single species of Plasmodium detected in the salivary glands, 29 (25.9%) had 1 or more additional species detected in the midgut, indicating a high potential for multiple infections. Similar proportions of Plasmodium species were found in An. gambiae s.l. and An. funestus.