Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
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DOI：
10.1590/s0074-02762010000600018
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发表时间：
2010-09-01
期刊：
影响因子：
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通讯作者：
Macedo, Andrea M
中科院分区：
文献类型：
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作者：
Andrade, Luciana O;Galvão, Lúcia MC;Macedo, Andrea M
我们以前已经证明了选择有利于JG株克氏锥虫在BALB/c小鼠的心脏，慢性感染的单克隆JG株和克隆的哥伦比亚株，Col1.7G2的平等的混合物。为了评估细胞侵袭效率是否驱动了这种选择，我们使用这些相同的T.克鲁兹人与预期相反，Col1.7G2寄生虫侵入心脏细胞培养物的数量高于JG寄生虫;然而，JG寄生虫的细胞内增殖比Col1.7G2寄生虫更有效。这种现象仅在心肌细胞中观察到，而在培养的Vero细胞中没有观察到。双重感染（Col1.7G2 + JG）显示相似的结果。即使入侵可能会影响组织选择，我们的数据强烈表明，细胞内的发展是重要的，以确定寄生虫的组织向性。
We have previously demonstrated selection favoring the JG strain of Trypanosoma cruzi in hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.