Significance of EGFR protein expression and gene amplification in non-small cell luna carcinoma
Significance of EGFR protein expression and gene amplification in non-small cell luna carcinoma
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DOI：
10.1309/h5uw6cpcwwc92241
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发表时间：
2006-06-01
影响因子：
3.5
通讯作者：
Yousem, Samuel A.
中科院分区：
文献类型：
--
作者：
Dacic, Sanja;Flanagan, Melina;Yousem, Samuel A.
应用免疫组织化学方法和荧光原位杂交技术检测199例初诊和手术治疗的非小细胞肺癌(NSCLC)组织中表皮生长因子受体(EGFR)蛋白的表达，并与临床病理结果对照。EGFR蛋白在鳞癌中的表达(17例[26.2%])明显高于腺癌(14例[11.1%]；P=.0076)，且与EGFR扩增有关(8例[14.5%]对4例[3.6%]；P=.0208)。分化不良与较高的平均每细胞EGFR基因拷贝数(平均4.18；P=.0322)和较高的EGFR/7号染色体比率(平均，1.84；P=.0324)有关。无疾病患者的EGFR基因拷贝数较高(平均为4.196，P=0.0163)。鳞癌的EGFR/7号染色体比值高于腺癌(平均为1.95比1.47；P=.0324)，尤其是T1肿瘤(平均为1.79；P=.0243)。统计分析未能显示早期非小细胞肺癌的预后与EGFR蛋白表达和基因扩增之间的相关性。在大多数病例中，EGFR蛋白的表达与基因扩增是分离的，尽管在SM的一个子集中存在良好的相关性。
We evaluated epidermal growth factor receptor (EGFR) protein expression by immunohistochemical analysis and EGFR gene amplification by fluorescence in situ hybridization in 199 consecutive newly diagnosed and surgically treated patients with primary non-small cell lung carcinoma (NSCLC) and correlated results with clinicopathologic findings. EGFR protein expression was more common in squamous cell carcinoma (SCC; 17 [26.2%]) than in adenocarcinoma (14 [11.1%]; P =.0076) and more frequently associated with EGFR amplification (8 [14.5%] vs 4 [3.6%] cases; P =.0208). Poor differentiation was associated with a higher average number of EGFR gene copies per cell (mean, 4.18; P =.0322) and a higher EGFR/chromosome 7 ratio (mean, 1.84; P =.0324). NO disease showed a higher number of EGFR gene copies (mean, 4.196; P =.0163). SCCs demonstrated a higher EGFR/chromosome 7 ratio than adenocarcinomas (mean, 1.95 vs 1.47; P =.0324), particularly T1 tumors (mean, 1.79; P =.0243). Statistical analysis failed to show correlation between outcome and EGFR protein expression and gene amplification in early NSCLC. EGFR protein expression was uncoupled from gene amplification in most cases, although good correlation occurred in a subset of SM.