Gene-Loaded Nanoparticle-Coated Sutures Provide Effective Gene Delivery to Enhance Tendon Healing
Gene-Loaded Nanoparticle-Coated Sutures Provide Effective Gene Delivery to Enhance Tendon Healing
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载有基因的纳米颗粒涂层缝线提供有效的基因传递以增强肌腱愈合
DOI：
10.1016/j.ymthe.2019.05.024
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发表时间：
2019
影响因子：
12.4
通讯作者：
Tang Jin Bo
中科院分区：
文献类型：
--
作者：
Zhou You Lang;Yang Qian Qian;Yan Ying Ying;Zhang Luzhong;Wang Qiu Hong;Ju Fei;Tang Jin Bo
如何加速肌腱愈合仍是临床上的一个挑战。在这项研究中，一种缝线携带纳米粒子/pEGFP-碱性成纤维细胞生长因子（bFGF）和pEGFP-血管内皮生长因子A（VEGFA）复合物的开发，转移到受伤的肌腱组织的生长因子基因，以促进愈合。聚多巴胺修饰的缝线可以均匀且紧密地吸附纳米颗粒/质粒复合物。肌腱组织缝合后，纳米颗粒/质粒复合物仍然存在于缝合线表面。此外，我们发现递送到肌腱组织中的纳米颗粒/质粒复合物可以从缝线扩散到肌腱组织，并有效地将基因导入肌腱细胞，显著增加肌腱组织中生长因子的表达。最后，生物力学测试表明，纳米粒子/pEGFP-bFGF和pEGFP-VEGFA复合物涂层缝线能够显著提高修复肌腱的极限强度，尤其是在术后4周。与相应的未修饰缝线相比，两种纳米颗粒/质粒复合物涂层缝线分别使Ethilon和PDS II的屈肌腱愈合强度显著增加了3.7倍和5.8倍。在屈肌腱损伤模型中，术后6周，与对照缝线相比，纳米颗粒/质粒复合物涂层缝线可显著增加肌腱的滑动位移，抑制粘连形成。这些结果表明，这种纳米颗粒/质粒复合物包被的缝合线是一种有前途的工具，用于治疗受伤的肌腱。
How to accelerate tendon healing remains a clinical challenge. In this study, a suture carrying nanoparticle/pEGFP-basic fibroblast growth factor (bFGF) and pEGFP-vascular endothelial growth factor A (VEGFA) complexes was developed to transfer the growth factor genes into injured tendon tissues to promote healing. Polydopamine-modified sutures can uniformly and tightly absorb nanoparticle/plasmid complexes. After tendon tissues were sutured, the nanoparticle/plasmid complexes still existed on the suture surface. Further, we found that the nanoparticle/plasmid complexes delivered into tendon tissues could diffuse from sutures to tendon tissues and effectively transfect genes into tendon cells, significantly increasing the expression of growth factors in tendon tissues. Finally, biomechanical tests showed that nanoparticle/pEGFP-bFGF and pEGFP-VEGFA complex-coated sutures could significantly increase the ultimate strengths of repaired tendons, especially at 4 weeks after operation. Two kinds of nanoparticle/plasmid complex-coated sutures significantly increased flexor tendon healing strength by 3.7 times for Ethilon and 5.8 times for PDS II, respectively, compared with the corresponding unmodified sutures. In the flexor tendon injury model, at 6 weeks after surgery, compared with the control suture, the nanoparticle/plasmid complex-coated sutures can significantly increase the gliding excursions of the tendon and inhibit the formation of adhesion. These results indicate that this nanoparticle/plasmid complex-coated suture is a promising tool for the treatment of injured tendons.