D8/17 and CD19 expression on lymphocytes of patients with acute rheumatic fever and Tourette's disorder.
D8/17 and CD19 expression on lymphocytes of patients with acute rheumatic fever and Tourette's disorder.
复制标题
急性风湿热和抽动秽语病患者淋巴细胞上 D8/17 和 CD19 的表达。
DOI：
10.1128/cdli.11.2.330-336.2004
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Hill,HarryR
中科院分区：
文献类型：
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作者：
Weisz,JulieL;McMahon,WilliamM;Moore,JillC;Augustine,NancyH;Bohnsack,JohnF;Bale,JamesF;Johnson,MichaelB;Morgan,JubelF;Jensen,Jenise;Tani,LloydY;Veasy,LGeorge;Hill,HarryR
D8/17是一种在B淋巴细胞上发现的同种异体抗原，据报道在易患风湿热的患者中升高，并可能与自身免疫类型的神经精神疾病有关。链球菌相关的儿童自身免疫性神经精神障碍模型是一组儿童的发病机制的假定模型，这些儿童的强迫症和抽动秽语障碍（TD）症状是突然的，可能由A组链球菌感染引发。作为该模型的检验，我们通过流式细胞术检测了TD和急性风湿热（ARF）患者的B细胞上的D8/17水平沿着正常对照的B细胞上的D8/17水平。我们已经使用了几种不同的D8/17抗体沿着制备物以及多种二抗，但是不能显示与ARF或TD中D8/17阳性、CD 19阳性B细胞百分比升高相关。然而，我们确实发现，与正常对照组相比，ARF和TD患者中CD 19阳性B细胞的百分比显著升高。然而，A组链球菌性咽炎患者的CD 19 B细胞百分比也升高。这些研究未能证实测定ARF患者或我们的TD患者样本中表达D8/17同种异体抗原的B细胞百分比的效用。相反，ARF和TD患者以及A组链球菌性咽炎患者中CD 19阳性B细胞的百分比显著升高，表明炎症和/或自身免疫在这些疾病的发病机制中起作用。
D8/17, an alloantigen found on B lymphocytes, has been reported to be elevated in patients susceptible to rheumatic fever and may be associated with autoimmune types of neuropsychiatric disorders. The pediatric-autoimmune-neuropsychiatric-disorders-associated-with-streptococci model is a putative model of pathogenesis for a group of children whose symptoms of obsessive-compulsive disorder and Tourette's disorder (TD) are abrupt and may be triggered by an infection with group A streptococci. As a test of this model, we have examined D8/17 levels on the B cells of patients with TD and acute rheumatic fever (ARF) along with those on the B cells of normal controls by flow cytometry. We have utilized several different preparations of D8/17 antibody along with a variety of secondary antibodies but have been unable to show an association with an elevated percentage of D8/17-positive, CD19-positive B cells in either ARF or TD. We did find, however, that the percentages of CD19-positive B cells in ARF and TD patients were significantly elevated compared to those in normal controls. Group A streptococcal pharyngitis patients also had an elevated percentage of CD19 B cells, however. These studies failed to confirm the utility of determining the percentage of B cells expressing the D8/17 alloantigen in ARF patients or our sample of TD patients. In contrast, the percentage of CD19-positive B cells was significantly elevated in ARF and TD patients, as well as group A streptococcal pharyngitis patients, suggesting a role for inflammation and/or autoimmunity in the pathogenesis of these disorders.