TAXONOMY AND PHYLOGENY OF INDUSTRIAL SOLVENT-PRODUCING CLOSTRIDIA
TAXONOMY AND PHYLOGENY OF INDUSTRIAL SOLVENT-PRODUCING CLOSTRIDIA
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DOI：
10.1099/00207713-45-4-693
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发表时间：
1995-10-01
期刊：
影响因子：
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通讯作者：
JONES, DT
中科院分区：
文献类型：
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作者：
KEIS, S;BENNETT, CF;JONES, DT
采用生物分型和DNA指纹分析相结合的方法对55株产溶剂梭菌进行了系统的研究，其中大多数梭菌目前被归类为乙酰丁酸梭菌，生物分型方法包括利福平药敏试验、细菌素分型、酸性噬菌体分型。55株菌株的生物型与DNA指纹图谱具有较好的相关性，可将其划分为9个类群，9个类群的DNA指纹图谱差异较大，但在每一类群内均表现出较高的相似性。为了确定9个群体的系统发育关系，我们进行了16S rRNA基因序列分析。对16S rRNA基因830 ~ 1383位（大肠杆菌编号）对应的部分序列进行比较分析，结果表明9个生物型群可组装为4个分类群。测定这些菌株的16S rRNA全序列。系统发育分析表明，解淀粉型菌株C. acetobutylicum ATCC 824（分类群I）与解糖型菌株C. acetobutylicum NCP 262、“Clostridium saccharoperbutylacetonicum”N1-4和C. acetobutylicum NCIMB 8052(T) （T =型菌株）仅具有亲缘关系（序列相似性为90 ~ 90.5%），属分类群II、III和IV的菌株分别为C. acetobutylicum NCP 262、C. acetobutylicum NCIMB 8052(T)；亲缘关系密切（序列相似度为98.2% ~ 98.9%）。C. acetobutylicum NCIMB 8052(T)与贝氏梭菌（Clostridium beijerinckii）的相似性为100%。对产糖溶剂菌株的重新分类是必要的，并讨论了四个分类群的可能名称。
We performed a systematic study of 55 solvent-producing clostridial strains, the majority of which are currently classified as Clostridium acetobutylicum strains, by using a combination of biotyping and DNA fingerprint analysis, The biotyping procedures used included rifampin susceptibility testing, bacteriocin typing, acid bacteriophage typing. The 55 strains examined exhibited a good correlation between their biotypes and DNA fingerprints, which allowed us to divide them into nine groups, The DNA fingerprints of the nine groups differed markedly, but within each group the DNA fingerprints exhibited a high level of similarity. To determine the phylogenetic relationships of the nine groups, we performed a 16S rRNA gene sequence analysis. The results of a comparative analysis of the partial sequence corresponding to positions 830 to 1383 (Escherichia coli numbering) of the 16S rRNA gene indicated that the nine biotype groups could be assembled into four taxonomic groups. The complete 16S rRNA sequences of strains representing these groups were determined. Our phylogenetic analysis revealed that the amylolytic type strain C. acetobutylicum ATCC 824 (taxonomic group I) was only distantly related to the saccharolytic strains belonging to taxonomic groups II, III, and (levels of sequence similarity, 90 to 90.5%), The strains belonging to taxonomic groups II, III, and IV, represented by C. acetobutylicum NCP 262, ''Clostridium saccharoperbutylacetonicum'' N1-4, and C. acetobutylicum NCIMB 8052(T) (T = type strain), respectively, were closely related (levels of sequence similarity, 98.2 to 98.9%). C. acetobutylicum NCIMB 8052(T) exhibited a level of similarity of 100% with the type strain of Clostridium beijerinckii. Reclassification of the saccharolytic solvent-producing strains is necessary, and possible names for the four taxonomic groups are discussed.