Regulation and function of the terminal gap gene huckebein in the Drosophila blastoderm.
Regulation and function of the terminal gap gene huckebein in the Drosophila blastoderm.
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DOI：
10.1387/ijdb.8735925
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发表时间：
1996-02
期刊：
影响因子：
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通讯作者：
Gunter Bronner;H. Jackle
中科院分区：
文献类型：
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作者：
Gunter Bronner;H. Jackle
果蝇的模式形成涉及一系列的母体和合子因子，这些因子在胚盘胚胎中受到空间限制。在这里，我们表明，果蝇基因huckebein（hkb），一个成员的差距基因类的分割基因，不仅需要在胚胎的末端区域的分割抑制，但也空间限制胚层形成在原肠胚形成的开始。hkb编码一种Sp1/egr样锌指蛋白，可能是一种转录因子。在hkb突变体中它的缺失导致外胚层和中胚层原基以内胚层原基为代价而扩展，而内胚层原基在hkb的无效等位基因中完全缺失。相反，hkb的异位表达抑制了主要原肠胚形成折叠的形成，从而产生了中胚层，并阻止了躯干区域外胚层的正常分割。
Pattern formation in Drosophila involves a cascade of maternal and zygotic factors which are spatially restricted in the blastoderm embryo. Here we show that the Drosophila gene huckebein (hkb), a member of the gap gene class of segmentation genes, is not only required for suppression of segmentation in the terminal regions of the embryo but also to spatially restrict germ layer formation at the beginning of gastrulation. hkb encodes a Sp1/egr-like zinc finger protein, likely to be a transcription factor. Its absence in hkb mutants causes the ectodermal and mesodermal primordia to expand at the expense of endoderm anlagen, which are completely absent in null alleles of hkb. Conversely, ectopic expression of hkb inhibits the formation of the major gastrulation fold which gives rise to the mesoderm and prevents normal segmentation in the ectoderm of the trunk region.