Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with xanthydrol
Determination of urea using high-performance liquid chromatography with fluorescence detection after automated derivatisation with xanthydrol
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DOI：
10.1016/j.chroma.2007.05.085
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发表时间：
2007-08-17
影响因子：
4.1
通讯作者：
Barnett, Neil W.
中科院分区：
文献类型：
--
作者：
Clark, Shona;Francis, Paul S.;Barnett, Neil W.
描述了一种高效液相色谱（HPLC）测定尿素的方法，该方法结合了黄原醇（9h -黄原醇-9-醇）的自动衍生化。与传统的测定尿素的黄原醇方法不同，该方法涉及沉淀二黄原脲（N,N '-二- 9h -黄原-9-脲），该方法中采用的衍生过程产生N- 9h -黄原-9-脲，其保留在溶液中，可以使用荧光检测(λ (ex) = 213 nin；λ (em) = 308 nm)从干扰中分离出来。尿素的检出限为5 × 10(-8) M （0.003 mg L-1）。本方法适用于人、动物尿液及酒中尿素的测定。(c) 2007 Elsevier B.V.版权所有
A high-performance liquid chromatography (HPLC) method for the determination of urea that incorporates automated derivatisation with xanthydrol (9H-xanthen-9-ol) is described. Unlike the classic xanthydrol approach for the determination of urea, which involves the precipitation of dixanthylurea (N,N '-di-9H-xanthen-9-ylurea), the derivatisation procedure employed in this method produces N-9H-xanthen-9-ylurea, which remains in solution and can be quantified using fluorescence detection (lambda(ex) = 213 nin; lambda(em) = 308 nm) after chromatographic separation from interferences. The limit of detection for urea was 5 x 10(-8) M (0.003 mg L-1). This method was applied to the determination of urea in human and animal urine and in wine. (c) 2007 Elsevier B.V. All rights reserved.