Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen
Effects of phosphoric acid and glutaraldehyde-HEMA on dentin collagen
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DOI：
10.1034/j.1600-0722.2001.00088.x
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发表时间：
2001-10-01
影响因子：
1.9
通讯作者：
Yamauchi, M
中科院分区：
文献类型：
--
作者：
Ritter, AV;Swift, EJ;Yamauchi, M
本研究的目的是评估磷酸 (PA) 和专有的戊二醛-HEMA 水溶液（Gluma Desensitizer.GD）对牙本质胶原蛋白的影响。脱矿牛牙本质胶原标本用 37% 或 50% PA 处理 1 分钟或 5 分钟。另外一组样本用 37% PA 处理 1 分钟，然后用 GD 处理 1 分钟。所有样品均用蒸馏水洗涤、冻干、用标准化 (NaBH4)-H-3 还原、用 6 M HCl 水解并进行氨基酸和交联分析。结果表明，在测试条件下用 PA 处理脱矿牙本质并没有显着改变胶原蛋白交联。经 GD 处理的样品显示游离赖氨酸 (Lys) 和羟基赖氨酸 (Hyl) 残基减少，并且胶原蛋白可还原交联水平降低。此外，通过高效液相色谱（HPLC）分析检测到了未鉴定的可还原化合物。这些化合物可能源自GD衍生的醛和胶原蛋白的Lys/Hyl之间形成的交联。研究结果表明，PA 处理不会显着影响牙本质胶原蛋白氨基酸和交联组成，而 GD 处理会影响牙本质胶原蛋白氨基酸和交联组成。
The purpose of this study was to evaluate the effects of phosphoric acid (PA) and a proprietary glutaraldehyde-HEMA aqueous solution (Gluma Desensitizer. GD) on dentin collagen. Specimens of demineralized bovine dentin collagen were treated with either 37% or 50% PA for I or 5 min. An additional set of specimens was treated with 37% PA for I min followed by GD for I min. All specimens were washed with distilled water, lyophilized, reduced with standardized (NaBH4)-H-3, hydrolyzed with 6 M HCl and subjected to amino acid and cross-link analyses. The results demonstrated that the treatment of demineralized dentin with PA under the conditions tested did not significantly alter the collagen cross-links. The GD-treated samples showed reduction of free lysine (Lys) and hydroxylysine (Hyl) residues, as well as a decrease in the levels of collagen reducible cross-links. In addition, unidentified reducible compounds were detected by high-performance liquid chromatography (HPLC) analysis. These compounds may be derived from cross-links formed between GD-derived aldehyde and Lys/Hyl of collagen. The findings indicate that PA treatment does not significantly affect dentin collagen amino acid and cross-link composition, and that GD treatment affects dentin collagen amino acid and cross-link composition.