Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser
Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser
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DOI：
10.1103/physrevlett.114.098102
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发表时间：
2015-03-02
影响因子：
8.6
通讯作者：
Hajdu, Janos
中科院分区：
文献类型：
--
作者：
Ekeberg, Tomas E;Svenda, Martin;Hajdu, Janos
我们提出了一个概念验证的三维重建的巨型mimivirus粒子从实验测量的衍射图案从x射线自由电子激光。三维成像需要将许多二维图案组装成内部一致的傅立叶体积。由于每个颗粒在暴露于X射线脉冲时随机取向，因此必须单独从衍射数据中检索相对取向。我们实现了这一点的扩展，最大化和压缩算法的修改版本，并使用新的方法验证我们的结果。
We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.