Identification of defined sequences in domain V of E. coli 23S rRNA in the 50S subunit accessible for hybridization with complementary oligodeoxyribonucleotides.
Identification of defined sequences in domain V of E. coli 23S rRNA in the 50S subunit accessible for hybridization with complementary oligodeoxyribonucleotides.
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鉴定可与互补寡脱氧核糖核苷酸杂交的 50S 亚基中大肠杆菌 23S rRNA 的结构域 V 中的确定序列。
DOI：
10.1093/nar/16.4.1603
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发表时间：
1988
影响因子：
14.9
通讯作者：
Hill,WE
中科院分区：
文献类型：
--
作者：
Marconi,RT;Hill,WE
区域V中特定序列的可达性。对coli23S rRNA 50S亚基上的互补寡脱氧核糖核酸（cDNA）进行了研究。用放射性标记的cDNA探针与50S亚基孵育，然后用硝化纤维素膜过滤反应混合物，并通过液体闪烁计数来测量结合的放射性标记cDNA探针，以确定亚基参与复合物形成的表观百分比。利用RNase h酶切RNA/DNA异源双工，确定了杂交位点。结果表明，单链序列2058 ~ 2062、2448 ~ 2454、2467 ~ 2483和2497 ~ 2505可与cDNA探针杂交。与2455-2461配对的碱基2489-2496也可以进行杂交。
The accessibility of specific sequences in domain V ofE. coli23S rRNA in the 50S subunit to complementary oligodeoxyribonucleotides (cDNA) has been investigated. The apparent percentage of subunlts engaged in complex formation was determined by incubation of radiolabeled cDNA probe with 50S subunits, followed by nitrocellulose membrane filtration of the reaction mixtures and measurement of the bound radiolabeled cDNA probes by liquid scintillation counting of the filters. The site(s) of hybridization were determined by digestion of the RNA in the RNA/DNA heteroduplex by RNase H.The results of this study indicated that single-stranded sequences, 2058–2062, 2448–2454, 2467–2483, and 2497–2505 were available for hybridization to cDNA probes. Bases 2489–2496, which have been postulated to be base paired with 2455–2461 were also accessible for hybridization.