Phylogenetic lineages in Pseudocercospora.
Phylogenetic lineages in Pseudocercospora.
复制标题
伪cospora中的系统发育谱系。
DOI：
10.3114/sim0005
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发表时间：
2013-06-30
影响因子：
16.5
通讯作者：
Groenewald JZ
中科院分区：
文献类型：
--
作者：
Crous PW;Braun U;Hunter GC;Wingfield MJ;Verkley GJ;Shin HD;Nakashima C;Groenewald JZ
假尾孢菌是一种世界性的植物病原真菌大属，通常与叶斑病、果斑病以及植物寄主上的枯萎病有关。它们发生在干旱和潮湿的环境中，以及包括凉爽的温带、亚热带和热带地区在内的各种气候中。假尾孢霉现在被认为是一个独立的属，尽管以前被认为是Mycosphaerella的变形状态或具有Mycosphaerella样的线形。这项研究的目的是对一组选定的分离物的部分28S核糖体RNA基因进行测序，以解决假尾孢菌复合体的系统发育属限制。从这些数据中，共识别出14个支系，其中6个为Mycosphaerellaceae的聚类群。假尾孢霉S代表一个不同的分支，是Passalora eucalypti的姐妹，以及一个代表Scolecostigmina，Trobihora和Pallidocercospora gen的分支。Nov.，以前在Mycosphaerella heimii复合体中的分类群，其特征是分生孢子光滑，淡棕色，以及在琼脂中形成红色晶体。菌丝菌科的其他分支包括海桑属、小环孢霉属和副孢子属。假尾孢霉与褐孢子菌、Miuraea、Cercospora和SepVictoria一起生活在一个大的分支中。其他分支包括盘花科、龟壳科、枝孢子科，以及十字花科、木兰属、金鱼草属和山茶属。中心孢霉属(PhaemycoCentropora)是为适应加拿大分枝杆菌而引入的，该属与假尾孢属的主要区别在于其透明的菌丝、宽广的分生孢子点和菌落高度。对发生在33个国家115个属的146种假尾孢菌进行了寄主专化性研究。ITS、EF-1、α和ACT3个基因座的部分核苷酸序列数据表明，这些物种中的大多数是宿主特有的。在同一地理区域内，根据寄主、症状和一般形态确定的物种在系统发育上经常不同，这表明通常没有必要将欧洲和美国的名称应用于亚洲分类群，反之亦然。新属--棕囊孢霉属(Pallidocercospora Cous)、中心棕霉菌属(PhaemycoCentropora Cous)、新属H.D.Shin和U.Braun；新种--杜氏尾孢霉、B.Braun和H.D.Shin、Quercina小孢子虫、Verkley、Ampelopsis Cous、B.Braun和H.D.Shin、C.cerocospora cercidicola Cous、B.Braun和C.Nakash、Pseudocercospora crispans G.C.Hunter&Cast、Pseudocercospora Crocea Cous、U.Braun、G.C.Hunter&H.D.Shin、Pusocercospora haiweiensCous&X.Shin、Pseudocercospora humulicola、U.Braun&H.D.Shin、Pusocercospora inceralisG.C.Hunter&H.D.Shin。M.E.Palm&U.Braun，Pseudocercospora plectranthi G.C.Hunter，Cous，U.Braun&H.D.Shin，Pusocercospora Proteae Cous，Pseudocercospora puostigmina-Platani Cous，U.Braun&H.D.Shin，Pusocercospora pyracanthigena Cous，U.Braun&H.D.Shin，Pusocercospora ravenalicola G.C.Hunter&Cous，Pseudocercospora rhamnellaG.C.Hunter，H.D.Shin，U.Braun&Cous，Pseudocercospora chinigucena&U.Braun&H.D.Shin，Pusocercospora chinigucena&U.Braun&U.Pusocercosporella koreana Cous，U.Braun&H.D.Shin；新组合--掌上孢霉[Pallidocercospora acciigena(Cous&M.J.Wingf.)Cous&M.J.Wingf.、结晶性早熟孢霉Pallidocercospora Crystal allina(Cous&M.J.Wingf.)Cous&M.J.Wingf.、棕榈孢霉(Pallidocercospora heimii(Cous)Cous)、棕囊霉(Pallidocercospora heimioides(Cous&M.J.Wingf.)Cous&M.J.Wingf.、棕榈孢属Pallidocercospora holualoana(Cous，Joanne E.Taylor&M.E.Palm)Cous、科氏棕榈孢属Pallidocercospora konae(Cous，Joanne E.Taylor&M.E.Palm)Cous、不规则棕榈孢属Pallidoercospora irrulariramosa(Cous&M.J.Wingf.)Cous&M.J.Wingf.、圆顶圆孢霉Phaemycocentropora cantuariensis(E.S.Salmon&WorMald)Cous、H.D.Shin&U.Braun、假尾孢霉Pseudocercospora hakeae(U.Braun&Cous)U.Braun&Cous、白假尾孢霉Pseudocercospora leucadendri(Cooke)U.Braun&Cous、斯奈氏假尾孢霉Pseudocercospora snelliana(Reichert)U.Braun、H.D.Shin、C.Nakash.&Cous，Pseudocercosporella chaenomelis(Y.Suto)C.Nakash.，Cous，U.Braun&H.D.Shin，表型：表型--狭叶假尾孢霉Pseudocercospora angolensis(T.Carvalho&O.Mendes)Cew&U.Braun，花冠假尾霉Pseudocercospora araliae(Henn.)Deighton，Pseudocercospora cersidis-chinensis H.D.Shin&U.Braun，Pseudocercospora corylopsidis(Togashi&Katsuki)C.Nakash.&Tak.Kobay.，Pseudocercospora dovyalidis(Chupp&Doidge)Deighton，Pseudocercospora fukuokaensis(Chupp)X.J.Liu&Y.L.Guo，Pseudocercospora humuli(Hori)Y.L.Guo&X.J.Liu，Pseudocercospora kigelariae(Syd.)Cous&U.Braun，Pusocercospora lyoniae(Katsuki&Tak.Kobay。)Deighton、Pseudocercospora lythri H.D.Shin&U.Braun、Pseudocercospora sambuigena U.Braun、Cous&K.Schub.、Pseudocercospora stephanandrae(Tak.科比。&H.Horie)C.Nakash.&Tak.Kobay.，Pseudocercospora viburnigena U.Braun&Cous，Pseudocercosporella chaenomelis(Y.Suto)C.Nakash.，Cew，U.Braun&H.D.Shin，Xenostigmina zilleri(A.Funk)Cous；&Cif.)Deighton；新定型法--假尾孢霉Pusocercospora kigelariae(Syd.)Cous&U.Braun，金银花假尾霉Pseudocercospora Lonicericola(W.Yamam.)Deighton，Pseudocercospora zelkovae(Hori)X.J.Liu&Y.L.Guo.
Pseudocercospora is a large cosmopolitan genus of plant pathogenic fungi that are commonly associated with leaf and fruit spots as well as blights on a wide range of plant hosts. They occur in arid as well as wet environments and in a wide range of climates including cool temperate, sub-tropical and tropical regions. Pseudocercospora is now treated as a genus in its own right, although formerly recognised as either an anamorphic state of Mycosphaerella or having mycosphaerella-like teleomorphs. The aim of this study was to sequence the partial 28S nuclear ribosomal RNA gene of a selected set of isolates to resolve phylogenetic generic limits within the Pseudocercospora complex. From these data, 14 clades are recognised, six of which cluster in Mycosphaerellaceae. Pseudocercospora s. str. represents a distinct clade, sister to Passalora eucalypti, and a clade representing the genera Scolecostigmina, Trochophora and Pallidocercospora gen. nov., taxa formerly accommodated in the Mycosphaerella heimii complex and characterised by smooth, pale brown conidia, as well as the formation of red crystals in agar media. Other clades in Mycosphaerellaceae include Sonderhenia, Microcyclosporella, and Paracercospora. Pseudocercosporella resides in a large clade along with Phloeospora, Miuraea, Cercospora and Septoria. Additional clades represent Dissoconiaceae, Teratosphaeriaceae, Cladosporiaceae, and the genera Xenostigmina, Strelitziana, Cyphellophora and Thedgonia. The genus Phaeomycocentrospora is introduced to accommodate Mycocentrospora cantuariensis, primarily distinguished from Pseudocercospora based on its hyaline hyphae, broad conidiogenous loci and hila. Host specificity was considered for 146 species of Pseudocercospora occurring on 115 host genera from 33 countries. Partial nucleotide sequence data for three gene loci, ITS, EF-1α, and ACT suggest that the majority of these species are host specific. Species identified on the basis of host, symptomatology and general morphology, within the same geographic region, frequently differed phylogenetically, indicating that the application of European and American names to Asian taxa, and vice versa, was often not warranted. New genera - Pallidocercospora Crous, Phaeomycocentrospora Crous, H.D. Shin & U. Braun; New species - Cercospora eucommiae Crous, U. Braun & H.D. Shin, Microcyclospora quercina Crous & Verkley, Pseudocercospora ampelopsis Crous, U. Braun & H.D. Shin, Pseudocercospora cercidicola Crous, U. Braun & C. Nakash., Pseudocercospora crispans G.C. Hunter & Crous, Pseudocercospora crocea Crous, U. Braun, G.C. Hunter & H.D. Shin, Pseudocercospora haiweiensis Crous & X. Zhou, Pseudocercospora humulicola Crous, U. Braun & H.D. Shin, Pseudocercospora marginalis G.C. Hunter, Crous, U. Braun & H.D. Shin, Pseudocercospora ocimi-basilici Crous, M.E. Palm & U. Braun, Pseudocercospora plectranthi G.C. Hunter, Crous, U. Braun & H.D. Shin, Pseudocercospora proteae Crous, Pseudocercospora pseudostigmina-platani Crous, U. Braun & H.D. Shin, Pseudocercospora pyracanthigena Crous, U. Braun & H.D. Shin, Pseudocercospora ravenalicola G.C. Hunter & Crous, Pseudocercospora rhamnellae G.C. Hunter, H.D. Shin, U. Braun & Crous, Pseudocercospora rhododendri-indici Crous, U. Braun & H.D. Shin, Pseudocercospora tibouchinigena Crous & U. Braun, Pseudocercospora xanthocercidis Crous, U. Braun & A. Wood, Pseudocercosporella koreana Crous, U. Braun & H.D. Shin; New combinations - Pallidocercospora acaciigena (Crous & M.J. Wingf.) Crous & M.J. Wingf., Pallidocercospora crystallina (Crous & M.J. Wingf.) Crous & M.J. Wingf., Pallidocercospora heimii (Crous) Crous, Pallidocercospora heimioides (Crous & M.J. Wingf.) Crous & M.J. Wingf., Pallidocercospora holualoana (Crous, Joanne E. Taylor & M.E. Palm) Crous, Pallidocercospora konae (Crous, Joanne E. Taylor & M.E. Palm) Crous, Pallidoocercospora irregulariramosa (Crous & M.J. Wingf.) Crous & M.J. Wingf., Phaeomycocentrospora cantuariensis (E.S. Salmon & Wormald) Crous, H.D. Shin & U. Braun, Pseudocercospora hakeae (U. Braun & Crous) U. Braun & Crous, Pseudocercospora leucadendri (Cooke) U. Braun & Crous, Pseudocercospora snelliana (Reichert) U. Braun, H.D. Shin, C. Nakash. & Crous, Pseudocercosporella chaenomelis (Y. Suto) C. Nakash., Crous, U. Braun & H.D. Shin; Typifications: Epitypifications - Pseudocercospora angolensis (T. Carvalho & O. Mendes) Crous & U. Braun, Pseudocercospora araliae (Henn.) Deighton, Pseudocercospora cercidis-chinensis H.D. Shin & U. Braun, Pseudocercospora corylopsidis (Togashi & Katsuki) C. Nakash. & Tak. Kobay., Pseudocercospora dovyalidis (Chupp & Doidge) Deighton, Pseudocercospora fukuokaensis (Chupp) X.J. Liu & Y.L. Guo, Pseudocercospora humuli (Hori) Y.L. Guo & X.J. Liu, Pseudocercospora kiggelariae (Syd.) Crous & U. Braun, Pseudocercospora lyoniae (Katsuki & Tak. Kobay.) Deighton, Pseudocercospora lythri H.D. Shin & U. Braun, Pseudocercospora sambucigena U. Braun, Crous & K. Schub., Pseudocercospora stephanandrae (Tak. Kobay. & H. Horie) C. Nakash. & Tak. Kobay., Pseudocercospora viburnigena U. Braun & Crous, Pseudocercosporella chaenomelis (Y. Suto) C. Nakash., Crous, U. Braun & H.D. Shin, Xenostigmina zilleri (A. Funk) Crous; Lectotypification - Pseudocercospora ocimicola (Petr. & Cif.) Deighton; Neotypifications - Pseudocercospora kiggelariae (Syd.) Crous & U. Braun, Pseudocercospora lonicericola (W. Yamam.) Deighton, Pseudocercospora zelkovae (Hori) X.J. Liu & Y.L. Guo.