Expression of en in the Prosencephalon Heterotopically Transplanted into the Mesencephalon
Expression of en in the Prosencephalon Heterotopically Transplanted into the Mesencephalon
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en 在前脑中的表达异位移植到中脑
DOI：
10.1111/j.1440-169x.1992.00387.x
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发表时间：
1992
期刊：
影响因子：
4.6
通讯作者：
N. Itasaki
中科院分区：
文献类型：
--
作者：
Harukazu Nakamura;N. Itasaki
在 10 体节阶段左右移植并整合到中脑时，前脑的翼板分化为顶盖状结构。含有基因 en 的同源框在中脑中表达，从 7 体节阶段开始具有尾前级梯度。这里使用抗 en 抗体对中脑前脑移植物中 en 的表达进行了免疫组织化学研究。在鹌鹑和小鸡之间进行移植。结果表明，移植物在中脑尾部被抗 en 抗体染色。在吻侧部分，移植物不表达 en。当移植物不邻近宿主中脑的尾部时，它不表达en。本研究表明，当前脑移植物在中脑尾部表达 en 时，它会分化为顶盖样结构。
The alar plate of the prosencephalon differentiates into a tectum‐like structure when transplanted and integrated into the mesencephalon around 10‐somite stage. Homeobox containing gene, en, is expressed in the mesencephalon with caudorostral gradient from 7‐somite stage. Here expression of en in the prosencephalic transplant in the mesencephalon was studied immunohistochemically with anti‐en antibody. Transplantation was carried out between quail and chick. It was shown that the transplant was stained with anti‐en antibody at the caudal part of the mesencephalon. At the rostral part, the transplant did not express en. When the transplant was not contiguous to the caudal part of the host mesencephalon, it did not express en. The present study suggests that the prosencephalic transplant differentiates into the tectum‐like structure when it expresses en at the caudal part of the mesencephalon.