Experimental Nipah virus infection in pigs and cats
Experimental Nipah virus infection in pigs and cats
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DOI：
10.1053/jcpa.2001.0532
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发表时间：
2002-02-01
影响因子：
0.8
通讯作者：
Hyatt, AD
中科院分区：
文献类型：
--
作者：
Middleton, DJ;Westbury, HA;Hyatt, AD
来自马来西亚的发热性脑炎爆发的尼帕病毒的人分离株与猪的疾病现场爆发一致，用于经口或皮下感染8头6周龄猪和2只猫口鼻感染。在猪中，该病毒引起的呼吸和神经系统综合征与在马来西亚猪中观察到的一致。并非所有猪均显示临床体征，但从临床和亚临床感染动物的鼻和口咽中均回收到尼帕病毒。很容易证明，接触猪的自然感染似乎是急性和自限性的。接种猪和接触猪均发生亚临床感染。在猫中也产生了呼吸系统和神经系统疾病，从尿液和口咽前部回收病毒。尼帕病毒在猫中诱导的临床和病理学综合征与亨德拉病毒感染在该物种中相关的临床和病理学综合征相当，但在尼帕病毒致死性感染中，呼吸道上皮广泛炎症，与病毒抗原的存在相关。如本研究中在猪和猫中观察到的，病毒通过鼻咽散毒不是猫和马实验性亨德拉病毒感染的早期报告的常规特征。研究结果表明，尼帕病毒可能通过呼吸道和口咽分泌物在猪之间传播。(C)2002 Elsevier Science Ltd.
A human isolate of Nipah virus from an outbreak of febrile encephalitis in Malaysia that coincided with a field outbreak of disease in pigs was used to infect eight 6-week-old pigs orally or subcutaneously and two cats oronasally. In pigs, the virus induced a respiratory and neurological syndrome consistent with that observed in the Malaysian pigs. Not all the pigs showed, clinical signs, but Nipah virus as recovered from the nose and oropharynx of both clinically and sub-clinically infected animals. Natural infection of in-contact pigs, which was readily demonstrated, appeared to be acute and self-limiting. Subclinical infectious occurred in both inoculated and in-contact pigs. Respiratory and neurological disease was also produced in the cats, with recovery of virus from urine as well as front the oropharynx. The clinical and pathological syndrome induced by Nipah virus in cats was comparable with that associated with Hendra virus infection in this species, except that in fatal infection with Nipah virus there was extensive inflammation of the respiratory epithelium, associated with the presence of viral antigen. Viral shedding via the nasopharynx, as observed in pigs and cats in the present study, was not a regular feature of earlier reports of experimental Hendra virus infection in cats and horses. the findings indicate the possibility of field transmission of Nipah virus between pigs via respiratory and oropharyngeal secretions. (C) 2002 Elsevier Science Ltd.