Identification of leptin in human saliva
Identification of leptin in human saliva
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DOI：
10.1210/jc.86.11.5234
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发表时间：
2001-11-01
影响因子：
5.8
通讯作者：
Dötsch, J
中科院分区：
文献类型：
--
作者：
Gröschl, M;Rauh, M;Dötsch, J
瘦素主要在脂肪组织中产生，但最近也在胃粘膜中发现。研究表明，口服瘦素可诱导啮齿动物脑干的神经元活动。本研究的目的是鉴定人类唾液中的这种激素，并检查唾液瘦素的产生和稳定性。我们通过RT-PCR证实了在唾液腺和口腔黏膜中瘦素的产生，免疫细胞化学证实了瘦素的储存，免疫细胞化学证实了瘦素的释放。色谱分析和免疫印迹证实了瘦素的身份。同时采集的唾液和血浆样品（n = 61）中瘦素浓度有很强的线性相关性（r(2) = 0.78）。刺激唾液流动可使总瘦素分泌增加3倍（P < 0.001）。至于瘦素在胃液中的稳定性，我们发现该肽在pH高于3.5时不被降解。此外，唾液瘦素在4℃下保持稳定至5 d。关于胃黏膜中存在瘦素受体，我们认为唾液瘦素可能是胃瘦素受体的配体。此外，唾液中瘦素的测定允许无创样本采集。
Leptin is produced predominantly in adipose tissue but has recently also been found in gastric mucosa. It has been shown that the oral application of leptin induces neuronal activity in the brain stem of rodents. The objective of the present study was to identify this hormone in human saliva and to examine the production and stability of salivary leptin. We have demonstrated production of leptin in salivary glands and oral mucosa by RT-PCR, its storage by immunocytochemistry, and the release of the peptide by RIA. Chromatographic analysis and immunoblotting confirmed the identity of leptin. There is a strong linear correlation (r(2) = 0.78) between leptin concentrations from simultaneously collected saliva and plasma samples (n = 61). Stimulation of saliva flow increases total leptin secretion up to 3-fold (P < 0.001). As to the stability of leptin in gastric fluid, we found the peptide was not degraded above pH 3.5. Additionally, salivary leptin remains stable up to 5 d at 4 C. With regard to the presence of leptin receptors in gastric mucosa, we suggest salivary leptin as being a possible ligand for gastric leptin receptors. Furthermore, the determination of leptin in saliva allows for noninvasive sample collection.