Zunongwangia pacifica sp. nov., isolated from surface seawater of the Western Pacific Ocean.
Zunongwangia pacifica sp. nov., isolated from surface seawater of the Western Pacific Ocean.
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DOI：
10.1099/ijsem.0.005606
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发表时间：
2022-11
影响因子：
2.8
通讯作者：
Yongqing Chen;Tianqi Liu;Qiliang Lai;Chunming Dong;Z. Shao
中科院分区：
文献类型：
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作者：
Yongqing Chen;Tianqi Liu;Qiliang Lai;Chunming Dong;Z. Shao
祖农王氏细菌是一群具有重要工业应用潜力和生态功能的海洋细菌。本研究从西太平洋菲律宾海盆表层海水中分离到一株革兰氏阴性、杆状、不能运动、严格需氧、亮黄色着色菌，命名为C2-37M9T。菌株C2-37M9T在10-44℃(最适28-30℃)、pH 6-9(PH 7)和0-12%的氯化钠(w/v；2-3%)中生长。基于16S rRNA基因序列的系统发育分析表明，该菌属于祖农旺亚属，与该属所有模式菌株的序列相似性为95.7-98.7%，其中与祖农旺亚的相似性最高(98.7%)。DNA-DNA数字杂交结果显示，C2-37M9T株与所有有效模式株的核苷酸同源性为27.5-32.3，核苷酸同源性为83.8-86.7，氨基酸同源性为86.9-89.0%。主要脂肪酸(>；5%)为：异C15：0、异C17：03-OH、正反C15：0、总和特征9(C16：0 10-甲基和/或异C17：1ω9c)、异C15：1G和总和特征3(C16：1ω6c和/或C16：1ω7c)。呼吸性对苯二酚主要为MK-6。极性脂为1个磷脂酰乙醇胺、2个未知糖脂、3个未知氨基脂和6个未知脂类。菌株C2-37M9T的基因组DNA G+C含量为36.7mol%。根据系统发育结果和基于基因组的亲缘关系指数，以及表型和基因特征，C2-37M9T菌株代表了Zunongwangia属的一个新种，命名为Zunongwangia Pacillia sp.11月是提议的。模式菌株为C2-37M9T(=MCCC M21534T=KCTC 82852T)。
Zunongwangia is a group of marine bacteria with important industrial application potential and ecological functions. In this study, a Gram-stain-negative, rod-shaped, non-motile, strictly aerobic and bright yellow pigmented bacterial strain within this genus, designated C2-37M9T, was isolated from a surface seawater sample from the Philippine Basin in the Western Pacific Ocean. Strain C2-37M9T grew at 10-44 °C (optimum, 28-30 °C), pH 6-9 (pH 7) and in the presence of 0-12 % NaCl (w/v; 2-3 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genus Zunongwangia and had 95.7-98.7 % sequence similarity to all type strains of this genus, with the highest value corresponding to Zunongwangia profunda (98.7 %). Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values between strain C2-37M9T and all valid type strains were 27.5-32.3, 83.8-86.7 and 86.9-89.0 %, respectively. The principal fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH, anteiso-C15 : 0, summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1 ω9c), iso-C15 : 1 G and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c). The predominant respiratory quinone was MK-6. The polar lipids were one phosphatidylethanolamine, two unknown glycolipids, three unidentified aminolipids and six unidentified lipids. The genomic DNA G+C content of strain C2-37M9T was 36.7 mol%. Based on phylogenetic results and genomic-based relatedness indices, as well as phenotypic and genotypic characteristics, strain C2-37M9T represents a novel species within the genus Zunongwangia, for which the name Zunongwangia pacifica sp. nov. is proposed. The type strain is C2-37M9T (=MCCC M21534T=KCTC 82852T).